20.500.12556/RUL-136995 Structural polymorphism of coiled-coils from the stalk domain of SARS-CoV-2 spike protein Spike trimer plays a key role in SARS-CoV-2 infection and vaccine development. It consists of a globular head and a flexible stalk domain that anchors the protein into the viral membrane. While the head domain has been extensively studied, the properties of the adjoining stalk are poorly understood. Here, we characterize the coiled-coil formation and thermodynamic stability of the stalk domain and its segments. We find that the N-terminal segment of the stalk does not form coiled-coils and remains disordered in solution. The C-terminal stalk segment forms a trimeric coiled-coil in solution, which becomes significantly stabilized in the context of the full-length stalk. Its crystal structure reveals a novel antiparallel tetramer coiled-coil with an unusual combination of a-d and e-a-d hydrophobic core packing. Structural analysis shows that a subset of hydrophobic residues stabilizes different coiled-coil structures: trimer, tetramer, and heterohexamer, underscoring a highly polymorphic nature of the SARS-CoV-2 stalk sequence. spike protein corona virus coiled-coil SARS-CoV-2 spike thermodynamics X-ray protein spike korona virus obvita vijačnica true false true Angleški jezik Slovenski jezik Članek v reviji 2022-05-27 13:08:59 2022-05-27 13:09:00 2022-09-15 04:14:34 0000-00-00 00:00:00 2022 0 0 11 str. iss. 3, art. e22199 Vol. 36 Mar. 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577.322 0892-6638 10.1096/fj.202101670R 97817859 The_FASEB_Journal_-_2022_-_ivi_-_Structural_polymorphism_of_coiled‐coils_from_the_stalk_domain_of_SARS‐CoV‐2_spike_protein.pdf The_FASEB_Journal_-_2022_-_ivi_-_Structural_polymorphism_of_coiled‐coils_from_the_stalk_domain_of_SARS‐CoV‐2_spike_protein.pdf 1 D5B27DA4679879C6816D8390698B0877 609a55fd12f36dd2b8ea70bf469184ec5b7e04ae35b10abaf4efbf881cff4296 14dbb52d-ddae-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=156836 https://faseb.onlinelibrary.wiley.com/doi/10.1096/fj.202101670R 1 459fdc85-ddad-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=156835 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0