20.500.12556/RUL-136914 Late blight resistance conferred by Rpi-Smira2/R8 in potato genotypes in vitro depends on the genetic background Potato production worldwide is threatened by late blight, caused by the oomycete Phytophthora infestans (Mont.) de Bary. Highly resistant potato cultivars were developed in breeding programs, using resistance gene pyramiding methods. In Sárpo Mira potatoes, five resistance genes (R3a, R3b, R4, Rpi-Smira1, and Rpi-Smira2/R8) are reported, with the latter gene assumed to be the major contributor. To study the level of late blight resistance conferred by the Rpi-Smira2/R8 gene, potato genotypes with only the Rpi-Smira2/R8 gene were selected from progeny population in which susceptible cultivars were crossed with Sárpo Mira. Ten R8 potato genotypes were obtained using stepwise marker-assisted selection, and agroinfiltration of the avirulence effector gene Avr4. Nine of these R8 genotypes were infected with both Slovenian P. infestans isolates and aggressive foreign isolates. All the progeny R8 genotypes are resistant to the Slovenian P. infestans isolate 02_07, and several show milder late blight symptoms than the corresponding susceptible parent after inoculation with other isolates. When inoculated with foreign P. infestans isolates, the genotype C571 shows intermediate resistance, similar to that of Sárpo Mira. These results suggest that Rpi-Smira2/R8 contributes to late blight resistance, although this resistance is not guaranteed solely by the presence of the R8 in the genome. potato potato genotype potato R8 genotypes in vitro infection late blight resistant cultivars oomicete Sárpo Mira Phytophthora infestans Solanum tuberosum L. Rpi-Smira2/R8 krompir krompirjeva plesen varstvo rastlin okužba rastlin oomicete genotipi krompirja odporne sorte true false true Angleški jezik Slovenski jezik Članek v reviji 2022-05-24 15:08:54 2022-05-24 15:08:57 2022-09-15 04:14:25 0000-00-00 00:00:00 2022 0 0 20 str. iss. 10 vol. 11 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2022-05-16 633 2223-7747 108979459 plants-11-01319-v2.pdf plants-11-01319-v2.pdf 1 A55510A966A882C6B086D164A6BDE0B8 354884b6ec9eeb7b62a34089ddca6d2b38faff8a042cffa87e70a8438f41cedd 5dbc3e76-db63-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=156724 https://www.mdpi.com/2223-7747/11/10/1319 1 8a5cb59b-db62-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=156723 Biotehniška fakulteta 0 0 0