20.500.12556/RUL-136428 Solubility of luteolin and other polyphenolic compounds in water, nonpolar, polar aprotic and protic solvents by applying FTIR/HPLC In recent years, flavonoids have become a highly researched topic due to their health beneficial effects. Since flavonoids’ solubility plays a significant role in their use in pharmaceutical, food, biological, and chemical areas, the determination of suitable solvents is crucial. Fourier transform infrared (FTIR) analysis was used to characterize functional groups of several flavonoids and phenolic compounds, namely luteolin, hesperidin, quercetin, naringenin, gallic acid and tannic acid. Concentration dependence on transmittance was evaluated for these compounds in ethanol. Afterwards, luteolin was chosen as a model flavonoid, with its concentration correlated with transmittance using 15 solvents with different polarities. Luteolin solubility was further corroborated with high-performance liquid chromatography (HPLC). These results shed light on using FTIR as a semi-quantitative method for the initial screening of solvents and the solubility of different compounds while saving time and solvents. Hence, HPLC would only be needed as a final step for the most promising solvents. luteolin dissolution fourier transform infrared-attenuated total reflectance (FTIR-ATR) spectroscopy fast quantification tool high-performance liquid chromatography (HPLC) biokemija FTIR flavonoidi Fourierjeva transformacija true false true Angleški jezik Slovenski jezik Članek v reviji 2022-05-03 15:23:42 2022-05-03 15:23:42 2022-09-15 04:13:52 0000-00-00 00:00:00 2021 0 0 15 str. iss. 11, art. 1952 Vol. 9 Nov. 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577 2227-9717 10.3390/pr9111952 85080835 processes-09-01952-v2.pdf processes-09-01952-v2.pdf 1 F0FB06A581643BB6123A60CA0B695ECE 8399111ed58efc6ba09bccb1eed5e856dff57d58ab6215c6c9f29a052b146bd0 80809a4e-cae4-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=156076 https://www.mdpi.com/2227-9717/9/11/1952 1 29c0a047-cae4-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=156075 Fakulteta za farmacijo 0 0 0