20.500.12556/RUL-136357 Isolation of chromatophores from brown trout (Salmo trutta) skin As new techniques for single-cell analysis are evolving, there is a growing need for streamlined and flexible protocols for single-cell suspension preparation and single-cell isolation. Based on previous research on the model species zebrafish (Danio rerio), a novel protocol for the isolation of chromatophores from brown trout (Salmo trutta) skin was developed and specific gene expression in chromatophore types evaluated with qPCR. This is the first report on a protocol for the isolation of chromatophores from the skin of salmonids. The method is simple and fast (taking about 90 min), requires no special equipment and is in line with the high ethical standards required in animal research, as no animals need to be sacrificed. In addition, skin biopsies can be taken from the same fish at multiple time points, allowing monitoring of chromatophore differentiation and their involvement in the formation of pigmentation patterns. The protocol should be effective for the vast majority of salmonid species due to similarities in skin morphology. melanophore erythrophore single cell qPCR ribe postrvi potočna postrv koža pigmentacija kromatofore izolacija metode qPCR true false true Angleški jezik Slovenski jezik Članek v reviji 2022-04-25 15:09:19 2022-04-25 15:09:19 2022-09-15 04:13:47 0000-00-00 00:00:00 2022 0 0 13 str. iss. 2, art. 72 Vol. 7 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579.2/.5 2410-3888 10.3390/fishes7020072 101735683 fishes-07-00072-v2.pdf fishes-07-00072-v2.pdf 1 78C6D357469EF68992D125837DE9A436 82f3070548fc34ed8cc0e39b28e03cd0e334c236a9c15d7656e0d5b11a26c9d9 8e54252e-c499-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=155962 https://www.mdpi.com/2410-3888/7/2/72 1 d7b09887-c498-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=155961 Biotehniška fakulteta 0 0 0