20.500.12556/RUL-136336 Systematic search for novel circulating biomarkers associated with extracellular vesicles in Alzheimerʹs disease combining literature screening and database mining approaches miRNAs play an important role in neurodegenerative diseases. Many miRNA-target gene interactions (MTI) have been experimentally confirmed and associated with Alzheimer’s disease (AD). miRNAs may also be contained within extracellular vesicles (EVs), mediators of cellular communication and a potential source of circulating biomarkers in body fluids. Therefore, EV-associated miRNAs (EV-miRNAs) in peripheral blood could support earlier and less invasive AD diagnostics. We aimed to prioritize EV-related miRNA with AD-related genes and to identify the most promising candidates for novel AD biomarkers. A list of unique EV-miRNAs from the literature was combined with a known set of AD risk genes and enriched for MTI. Additionally, miRNAs associated with the AD phenotype were combined with all known target genes in MTI enrichment. Expression in different sample types was analyzed to identify AD-associated miRNAs with the greatest potential as AD circulating biomarkers. Four common MTI were observed between EV-miRNAs and AD-associated miRNAs: hsa-miR-375–APH1B, hsa-miR-107–CDC42SE2, hsa-miR-375–CELF2, and hsa-miR-107–IL6. An additional 61 out of 169 unique miRNAs (36.1%) and seven out of 84 unique MTI (8.3%), observed in the body fluids of AD patients, were proposed as very strong AD-circulating biomarker candidates. Our analysis summarized several potential novel AD biomarkers, but further studies are needed to evaluate their potential in clinical practice. Alzheimer’s disease biomarker miRNA extracellular vesicles medicina Alzheimerjeva bolezen bioinformatika biomarkerji genetika miRNA zunajcelični vezikli true false true Angleški jezik Slovenski jezik Članek v reviji 2022-04-25 10:57:54 2022-04-25 10:57:55 2022-09-15 04:13:46 0000-00-00 00:00:00 2021 0 0 14 str. iss. 10, art. 946 Vol. 11 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616:575 2075-4426 10.3390/jpm11100946 77794051 jpm-11-00946.pdf jpm-11-00946.pdf 1 B13829B6F70FAEDB546B7DE467FBECC7 a9c9e46f16f6e38326d6f93af174926c2771d342e28f71af8a4ddeb3c34b88e2 ef1eda4e-c475-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=155921 https://www.mdpi.com/2075-4426/11/10/946 1 b8ce7044-c475-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=155920 Medicinska fakulteta Biotehniška fakulteta 0 0 0