20.500.12556/RUL-136335 Oral glucose load and human cutaneous microcirculation an insight into flowmotion assessed by wavelet transform Microcirculation in vivo has been assessed using non-invasive technologies such as laser Doppler flowmetry (LDF). In contrast to chronic hyperglycemia, known to induce microvascular dysfunction, the effects of short-term elevations in blood glucose on microcirculation are controversial. We aimed to assess the impact of an oral glucose load (OGL) on the cutaneous microcirculation of healthy subjects, quantified by LDF and coupled with wavelet transform (WT) as an interpretation tool. On two separate occasions, sixteen subjects drank either a glucose solution (75 g in 250 mL water) or water (equal volume). LDF signals were obtained in two anatomical sites (forearm and finger pulp) before and after each load (pre-load and post-load, respectively), in resting conditions and during post-occlusive reactive hyperemia (PORH). The WT allowed decomposition of the LDF signals into their spectral components (cardiac, respiratory, myogenic, sympathetic, endothelial NO-dependent). The OGL blunted the PORH response in the forearm, which was not observed with the water load. Significant differences were found for the cardiac and sympathetic components in the glucose and water groups between the pre-load and post-load periods. These results suggest that an OGL induces a short-term subtle microvascular impairment, probably involving a modulation of the sympathetic nervous system. diabetes mellitus microcirculation laser Doppler flowmetry wavelet transform oral glucose load flowmotion post-occlusive reactive hyperemia sladkorna bolezen mikrocirkulacija laserska Dopplerjeva flowmetrija true false true Angleški jezik Slovenski jezik Članek v reviji 2022-04-25 10:26:37 2022-04-25 10:26:40 2022-09-15 04:13:46 0000-00-00 00:00:00 2021 0 0 17 str. iss. 10, art. 953 Vol. 10 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 612 2079-7737 10.3390/biology10100953 83675651 biology-10-00953-v2.pdf biology-10-00953-v2.pdf 1 1810476C6D8E1667CCCAEA7136D038B4 94a7696ad2cf4690ac80bb4986f944a3a460deca66d33c0aef5bd5c5090db8b5 33cccc52-c474-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=155919 https://www.mdpi.com/2079-7737/10/10/953 1 5b489300-c471-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=155918 Medicinska fakulteta 0 0 0