20.500.12556/RUL-136333 Expression of cytokine-coding genes BMP8B, LEFTY1 and INSL5 could distinguish between ulcerative colitis and Crohn's disease Ulcerative colitis (UC) and Crohn’s disease (CD) are characterized by an imbalance between pro-inflammatory and anti-inflammatory cytokines, interfering with the resolution of inflammation. Due to the crucial role of cytokines, new insights into their profiles in UC and CD would help to improve our understanding of pathogenesis and enable the development of new treatment modalities. We provide an expression profile of cytokines in UC and CD, using bioinformatics approach, and experimental validation of expression of the selected genes. We retrieved data and analyzed the cytokine gene expression profiles of UC and CD. From ten genes with inverse expression, common to CD and UC, BMP8B, LEFTY1 and INSL5 were selected for gene expression experimental validation. Experimentally, BMP8B and INSL5 were down-regulated in both CD and UC but followed the bioinformatics trend. The expression of genes LEFTY1 and BMP8B was statistically significant when comparing UC and CD in colon and the expression of gene LEFTY1 showed statistical significance when CD in ileum and colon were compared. Using the bioinformatics approach and experimental validation, we found differences in expression profiles between UC and CD for INSL5, LEFTY1 and BMP8B. These three promising candidate genes need to be further explored at different levels, such as DNA methylation and protein expression, to provide more evidence on their potential diagnostic role in CD and UC. inflammatory bowel disease ulcerative colitis Crohn's disease inflammatory cytokines expression bioinformatics approach experimental validation vnetje črevesja ulcerozni kolitis Crohnova bolezen true false true Angleški jezik Slovenski jezik Članek v reviji 2022-04-25 09:54:35 2022-04-25 09:54:38 2022-09-15 04:13:46 0000-00-00 00:00:00 2021 0 0 14 str. iss. 10, art. 1477 Vol. 12 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616.3 2073-4425 10.3390/genes12101477 77672451 genes-12-01477.pdf genes-12-01477.pdf 1 DF898E1A55796DDD416888AEAF5D8748 3347dfcb5b35c527a725ad600583e9e07461707ac6a104e5244ab2f4bb46806c 40884fc9-c46d-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=155916 https://www.mdpi.com/2073-4425/12/10/1477 1 e289682f-c46c-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=155914 Medicinska fakulteta 0 0 0