20.500.12556/RUL-136195 Identification and characterization of Verticillium nonalfalfae-responsive microRNAs in the roots of resistant and susceptible hop cultivars MicroRNAs are 21- to 24-nucleotide-long, non-coding RNA molecules that regulate gene expression at the post-transcriptional level. They can modulate various biological processes, including plant response and resistance to fungal pathogens. Hops are grown for use in the brewing industry and, recently, also for the pharmaceutical industry. Severe Verticillium wilt caused by the phytopathogenic fungus Verticillium nonalfalfae, is the main factor in yield loss in many crops, including hops (Humulus lupulus L.). In our study, we identified 56 known and 43 novel miRNAs and their expression patterns in the roots of susceptible and resistant hop cultivars after inoculation with V. nonalfalfae. In response to inoculation with V. nonalfalfae, we found five known and two novel miRNAs that are differentially expressed in the susceptible cultivar and six known miRNAs in the resistant cultivar. Differentially expressed miRNAs target 49 transcripts involved in protein localization and pigment synthesis in the susceptible cultivar, whereas they are involved in transcription factor regulation and hormone signalling in the resistant cultivar. The results of our study suggest that the susceptible and resistant hop cultivars respond differently to V. nonalfalfae inoculation at the miRNA level and that miRNAs may contribute to the successful defence of the resistant cultivar. Humulus lupulus Verticillium nonalfalfae biotic stress microRNA high-throughput sequencing Verticillium nonalfalfae Humulus lupulus biotični stres mikro RNA sekvenciranje true false true Angleški jezik Slovenski jezik Članek v reviji 2022-04-19 10:16:08 2022-04-19 10:16:08 2022-09-15 04:13:31 0000-00-00 00:00:00 2021 0 0 18 str. iss. 9, art. 1883 Vol. 10 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 60 2223-7747 10.3390/plants10091883 77436931 plants-10-01883-v2.pdf plants-10-01883-v2.pdf 1 505BBE8C848030214AD7E43A5217F907 61381d28c736acb7bdda16eedaedea675e7f3f1adb308bf9d6aaa5b9c46fdfa4 4a81f3ec-bfb9-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=155712 https://www.mdpi.com/2223-7747/10/9/1883 1 e735c92f-bfb8-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=155711 Biotehniška fakulteta 0 0 0