20.500.12556/RUL-136159 Efflux pump inhibition and resistance modulation in Mycobacterium smegmatis by Peucedanum ostruthium and its coumarins Antibiotic resistance is a growing problem and may become the next major global health crisis if no timely actions are taken. Mycobacterial infections are widespread and, due to antibiotic resistance, also hard to treat and a major cause of mortality. Natural compounds have the potential to increase antibiotic effectiveness due to their resistance modulatory and antimicrobial effects. In this study, Peucedanum ostruthium extracts, fractions, and isolated compounds were investigated regarding their antimicrobial and resistance-modulatory effects as well as efflux pump inhibition in Mycobacterium smegmatis. P. ostruthium extracts were found to have anti-mycobacterial potential and resistance modulating effects on ethidium bromide activity. The major antibacterial effect was attributed to ostruthin, and we found that the more lipophilic the substrate, the greater the antimicrobial effect. Imperatorin caused potent modulatory effects by interfering with the action of the major LfrA efflux pump in M. smegmatis. The plant P. ostruthuim has a complex effect on M. smegmatis, including antibacterial, efflux pump inhibition, resistance modulation, and membrane permeabilization, and its major constituents, ostruthin and imperatorin, have a distinct role in these effects. This makes P. ostruthium and its coumarins promising therapeutics to consider in the fight against drug-resistant mycobacteria. Mycobacterium smegmatis efflux pump inhibitor Peucedanum ostruthium antibacterial properties ostruthin imperatorin ethidium bromide accumulation resistance modulation mikobakterije odpornost proti antibiotikom izlivne črpalke protimikrobne snovi true false true Angleški jezik Slovenski jezik Članek v reviji 2022-04-14 08:46:45 2022-04-14 08:46:46 2022-09-15 04:13:28 0000-00-00 00:00:00 2021 0 0 17 str. iss. 9, art. 1075 Vol. 10 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579.24/.26:547.9:615.3 2079-6382 10.3390/antibiotics10091075 75651587 antibiotics-10-01075.pdf antibiotics-10-01075.pdf 1 A10DDD3992C506434F62E280493AE5A2 c98543b4e0f3cc0964cfbd700c5daa4c074a49b7f0f8b5eeefbef348e674bb48 e2ccfb39-bbbe-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=155650 https://www.mdpi.com/2079-6382/10/9/1075 1 996ac83c-bbbe-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=155649 Biotehniška fakulteta 0 0 0