20.500.12556/RUL-136078 Conditional cooperativity in DNA minor-groove recognition by oligopeptides The recognition of specific DNA sequences in processes such as transcription is associated with a cooperative binding of proteins. Some transcription regulation mechanisms involve additional proteins that can influence the binding cooperativity by acting as corepressors or coactivators. In a conditional cooperativity mechanism, the same protein can induce binding cooperativity at one concentration and inhibit it at another. Here, we use calorimetric (ITC) and spectroscopic (UV, CD) experiments to show that such conditional cooperativity can also be achieved by the small DNA-directed oligopeptides distamycin and netropsin. Using a global thermodynamic analysis of the observed binding and (un)folding processes, we calculate the phase diagrams for this system, which show that distamycin binding cooperativity is more pronounced at lower temperatures and can be first induced and then reduced by increasing the netropsin or/and Na+ ion concentration. A molecular interpretation of this phenomenon is suggested. DNA recognition conditional cooperativity transcription regulation protein–DNA interactions ligand–DNA interactions distamycin netropsin thermodynamics of binding phase diagrams DNA folding prepoznavanje DNA pogojna kooperativnost regulacija transkripcije interakcije protein – DNA interakcije ligand – DNA distamicin netropsin termodinamika vezave fazni diagrami zvitje DNA true false true Angleški jezik Slovenski jezik Članek v reviji 2022-04-11 08:20:24 2022-04-11 08:20:27 2022-09-15 04:13:22 0000-00-00 00:00:00 2021 0 0 10 str. iss. 17, art. 5188 Vol. 26 Sep. 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577.323 1420-3049 10.3390/molecules26175188 87050755 RAZ_Lah_Jurij_2021.pdf RAZ_Lah_Jurij_2021.pdf 1 DE7A53F586AFC17B2CC5F54B4F7D6F3F 02d12f1724fb95fa48a47156879b8cf8534d54bde6d2ca585ecdb9c6ab107ee0 ad00e6a3-b95f-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=155526 https://www.mdpi.com/1420-3049/26/17/5188 0 6eb54102-b95f-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=155525 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0