20.500.12556/RUL-136021 Determination of biogenic amines in fresh fish and processed fish products using IC-MS/MS A new method was proposed for the determination of underivatized biogenic amines based on ion-exchange chromatography coupled with mass spectrometry detection. The method was applied to the analysis of 10 biogenic amines in fresh and processed fish products. The amines were extracted from muscle tissue with water without any additional derivative step or sample clean-up. Separation of biogenic amines was done by the IonPac (4 × 50 mm) column, applying a gradient eluent by mixing formic acid (2 mol L$^{−1}$) and Milli-Q water (formic acid concentration from 400 mM to 2 M). The results demonstrated a linear response in the range of 0.01 to 10 mg L$^{−1}$. The detection limits for the fish products ranged from 20 ng/g up to around 400 ng/g for histamine and putrescine, respectively. Spermidine and spermine showed significantly higher detection limits. This current method can be used for the determination of biogenic amines in both fresh and processed fish products for regulatory purposes and monitoring food-safety issues relating to these amines, particularly histamine. It is also a useful method for evaluation of other commercial analytical test kits and commonly used methods that are possibly affected by the food matrix due to processing or other drawbacks arising from the derivatization process. biogenic amines determination fish products histamine ion chromatography MS/MS biogeni amini določitev ribji izdelki histamin ionska kromatografija masna spektrometrija true false true Angleški jezik Slovenski jezik Članek v reviji 2022-04-07 08:02:57 2022-04-07 08:02:59 2022-09-15 04:13:14 0000-00-00 00:00:00 2021 0 0 14 str. iss. 8, art. 1746 Vol. 10 Aug. 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 543.544.14:543.51:664.95 2304-8158 10.3390/foods10081746 73813507 RAZ_Kocar_Drago_2021.pdf RAZ_Kocar_Drago_2021.pdf 1 C4F10BA15098386254AD151DB1A9767D 5c1fc9080ace10cf672d7c51270c44bf406a0213b486fbc524ffc4d9be236cb7 b1b0f757-b638-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=155443 https://www.mdpi.com/2304-8158/10/8/1746 0 56145e9c-b638-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=155441 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0