20.500.12556/RUL-135983 Insight into the antioxidant effect of fermented and non-fermented Spirulina water and ethanol extracts at the proteome level using a yeast cell model Spirulina is rich in various antioxidants and nutraceuticals and it has proven to be effective in the treatment of various pathological conditions. This study explores the antioxidant effect of fermented and non-fermented Spirulina extracts on the proteome level using the yeast Saccharomyces cerevisiae as a model organism. Yeast cells were treated with fermented Spirulina water extract (SV), non-fermented Spirulina water extract (NFV), fermented Spirulina ethanol extract (SE), and nonfermented Spirulina ethanol extract (NFE). Cell lysates were prepared, and label-free quantitative proteome analysis was performed. In SV, when compared to NFV samples, the levels of most differentially expressed proteins were upregulated. Alternatively, SE compared to NFE samples showed a significant downregulation for the majority of the analyzed proteins involved in different cellular processes. Additionally, a higher downregulation of stress response related proteins was observed in SE compared to NFE samples, while their abundance in SV samples increased compared to NFV. This study provided a global view, on a proteome level, of how cells cope with exogenous antioxidants and remodel their cellular processes to maintain metabolic and redox balance. Furthermore, it combined for the first time the analysis of different extract effect, including the contribution of lactic acid fermentation to the cell activity. Spirulina lactic acid fermentation Saccharomyces cerevisiae ethanol proteome antioxidant stress response mikroalge spirulina fermentacija antioksidanti proteomika true false true Angleški jezik Slovenski jezik Članek v reviji 2022-04-05 13:44:13 2022-04-05 13:44:14 2022-09-15 04:13:04 0000-00-00 00:00:00 2021 0 0 15 str. iss. 9, art. 1366 Vol. 10 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577 2076-3921 10.3390/antiox10091366 74719747 RAZ_Masten_Jasmina_2021.pdf RAZ_Masten_Jasmina_2021.pdf 1 F72643EFEC062E1284DC5C91C9DBAB85 b67482bf22a4e3b1a92ac66a5c886d366c8648362368b28a3cfc9fc1d9337da7 e5e1c4a8-b4d5-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=155339 https://www.mdpi.com/2076-3921/10/9/1366 0 c1464043-b4d5-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=155338 Biotehniška fakulteta 0 0 0