20.500.12556/RUL-135981 Stratified mucin-producing intraepithelial lesion (SMILE) of the uterine cervix high-risk HPV genotype predominance and p40 immunophenotype Stratified mucin-producing intraepithelial lesion (SMILE) is a rare high-grade cervical precancerous lesion designated a variant of adenocarcinoma in situ (AIS) in the WHO classification. We aimed to determine HPV genotypes, immunohistochemical phenotype and mucin presence in SMILE. Between 2010 and 2018, SMILE was diagnosed in 34 out of 6958 (0.5%) cervical biopsies, in 23 patients. Twenty-six tissue samples from twenty-one patients were available for further analysis, including 13 with SMILE alone, 12 with SIL and/or AIS and one with HSIL, AIS and endocervical adenocarcinoma. HPV genotyping was performed using the Seegene Anyplex II HPV 28 assay. Of the 26 samples, a single HPV genotype was identified in the majority of cases (n = 22), including 12/13 SMILEs associated with SIL/AIS. All but one were high-risk HPV genotypes (23/24; 96.8%). We identified seven different HPV genotypes, the most common being HPV16 (n = 10; 43.5%), HPV18 (n = 8, 34.8%) and HPV 31 (n = 5, 21.7%). All SMILEs showed a strong positive reaction to p16, CK7, CK19 and high Ki67 expression comparable to adjacent HSIL and/or AIS if present. SMILE showed variable mucin presence and p40-positive squamous differentiation suggesting phenotypic diversity in cervical precancerous lesions infected by single HPV. cervical precancerous lesion intraepithelial lesions HPV genotyping stratified mucin-producing intraepithelial lesions immunohistochemistry high-risk HPV types predrakava lezija materničnega vratu intraepitelijske lezije genotipizacija HPV true false true Angleški jezik Slovenski jezik Članek v reviji 2022-04-05 13:27:48 2022-04-05 13:27:55 2022-09-15 04:13:03 0000-00-00 00:00:00 2021 0 0 10 str. iss. 8, art. 2039 Vol. 10 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616 2073-4409 10.3390/cells10082039 72916483 RAZ_Strojan_Flezar_Margareta_2021.pdf RAZ_Strojan_Flezar_Margareta_2021.pdf 1 877AF263CE73B2657D2FBD40823C890D 409f59441c50053f11ebbc251abe027266987be6c22795bc86d42cd4020618bc 9fbdc9b1-b4d3-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=155334 https://www.mdpi.com/2073-4409/10/8/2039 1 678db95f-b4d3-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=155333 Medicinska fakulteta 0 0 0