20.500.12556/RUL-135965 TRIM28 selective nanobody reduces glioblastoma stem cell invasion Glioblastoma (GB), is the most common and aggressive malignant primary brain tumour in adults. Intra- and inter-tumour heterogeneity, infiltrative GB cell invasion and presence of therapy-resistant GB stem cells (GSCs) represent major obstacles to favourable prognosis and poor therapy response. Identifying the biomarkers of the most aggressive tumour cells and their more efficient targeting strategies are; therefore, crucial. Recently, transcription factor TRIM28 has been identified as a GB biomarker and, in this study, we have shown high expression of TRIM28 in GB and in low grade gliomas as well as higher expression in GSCs vs. differentiated GB cells, although in both cases not significant. We demonstrated significant in vitro inhibition of GB cells and GSCs invasiveness and spread in zebrafish brains in vivo by anti-TRIM28 selective nanobody NB237. TRIM28 was also enriched in GB (tumour) core and associated with the expression of stem cell genes, but was not prognostic for overall survival. However, based on the above results, we conclude that TRIM28 nanobody NB237 offers a new opportunity as a GB therapeutic tool. glioblastoma nanobody glioblastoma stem cells cell invasion transcription factor TRIM28 glioblastom TRIM28 nanoprotitelesa true false true Angleški jezik Slovenski jezik Članek v reviji 2022-04-05 09:19:49 2022-04-05 09:19:50 2022-09-15 04:12:59 0000-00-00 00:00:00 2021 0 0 16 str. iss. 17, art. 5141 Vol. 26 Aug. 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 620.3:620.1/.2 1420-3049 10.3390/molecules26175141 74227715 RAZ_Porcnik_Andrej_2021.pdf RAZ_Porcnik_Andrej_2021.pdf 1 695FE1C98BA264B22AB018FF6CB0DAAF 5928a4c5d2853ded71411b1f364da9ca0f9d0a6e9db564ade188a173deba4b62 0feb95b1-b4b1-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=155306 https://www.mdpi.com/1420-3049/26/17/5141 1 c081d193-b4b0-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=155305 Medicinska fakulteta Biotehniška fakulteta Fakulteta za kemijo in kemijsko tehnologijo 0 0 0