20.500.12556/RUL-135752 Legionella pneumophila—epidemiology and characterization of clinical isolates, Slovenia, 2006–2020 Legionella pneumophila is the causative agent of severe Legionnaires’ disease (LD). Although an increasing number of LD cases have been observed, published data from Slovenia are very limited and data on molecular epidemiology are even scarcer. The present retrospective study (2006–2020) reports the results of the microbiological diagnosis of LD, as well as the epidemiology and characterization of the Legionella clinical isolates. We tested urine samples from 15,540 patients with pneumonia symptoms for L. pneumophila infection by urine antigen test, of which 717 (4.6%) tested positive. Isolation of L. pneumophila was successfully performed from 88 clinical specimens, with 82 (93.2%) being identified as L. pneumophila sg 1 and six (6.8%) as L. pneumophila sg 2–14. Sequence-based typing (SBT) identified 33 different sequence types (STs), the most frequent being ST1 and ST23. Sequence type 1 mainly comprised isolates belonging to the Philadelphia subgroup, and ST23 mostly to Allentown/France. The standard SBT scheme, as well as Dresden phenotyping for L. pneumophila, presented a high diversity among isolates. Legionnaires' disease diagnosis epidemiology Legionella pneumophila typing cultivation legionarska bolezen diagnoza epidemiologija true false true Angleški jezik Slovenski jezik Članek v reviji 2022-03-30 13:03:00 2022-03-30 13:03:04 2022-09-14 04:14:59 0000-00-00 00:00:00 2021 0 0 11 str. iss. 7, art. 1201 Vol. 11 May 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616.9 2075-4418 10.3390/diagnostics11071201 69395715 RAZ_Kese_Darja_2021.pdf RAZ_Kese_Darja_2021.pdf 1 596BDC553C40CF2F3D786A970C46D6D2 d8d082497e9944c8491695af79d7754972537af3f8f527ed74ee58dbd8b1c989 614b59d9-b019-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=155026 https://www.mdpi.com/2075-4418/11/7/1201 1 f45455ea-b018-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=155025 Medicinska fakulteta 0 0 0