20.500.12556/RUL-135751 Basic methods for preparation of liposomes and studying their interactions with different compounds, with the emphasis on polyphenols Studying the interactions between lipid membranes and various bioactive molecules (e.g., polyphenols) is important for determining the effects they can have on the functionality of lipid bilayers. This knowledge allows us to use the chosen compounds as potential inhibitors of bacterial and cancer cells, for elimination of viruses, or simply for keeping our healthy cells in good condition. As studying those effect can be exceedingly difficult on living cells, model lipid membranes, such as liposomes, can be used instead. Liposomal bilayer systems represent the most basic platform for studying those interactions, as they are simple, quite easy to prepare and relatively stable. They are especially useful for investigating the effects of bioactive compounds on the structure and kinetics of simple lipid membranes. In this review, we have described the most basic methods available for preparation of liposomes, as well as the essential techniques for studying the effects of bioactive compounds on those liposomes. Additionally, we have provided details for an easy laboratory implementation of some of the described methods, which should prove useful especially to those relatively new on this research field. model lipid bilayers bioactive compounds membrane fluidity membrane kinetics essential methods for liposome/vesicle preparation bioaktivne spojine modelne lipidne membrane liposomi true false true Angleški jezik Slovenski jezik Članek v reviji 2022-03-30 12:50:59 2022-03-30 12:51:00 2022-09-14 04:14:59 0000-00-00 00:00:00 2021 0 0 20 str. iss. 12, art. 6547 Vol. 22 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577.352:547.56 1422-0067 10.3390/ijms22126547 67612675 RAZ_Sturm_Luka_2021.pdf RAZ_Sturm_Luka_2021.pdf 1 E8951768C6B3BA01E9D31D9D6CA41FBF 95fd6887b76dccefd5ea52c89707465737242f79242d406772eab0b613567e87 9741bca2-b017-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=155024 https://www.mdpi.com/1422-0067/22/12/6547 1 4843228c-b017-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=155023 Biotehniška fakulteta 0 0 0