20.500.12556/RUL-135742 Towards the well-tempered chloroplast DNA sequences With the development of next-generation sequencing technology and bioinformatics tools, the process of assembling DNA sequences has become cheaper and easier, especially in the case of much shorter organelle genomes. The number of available DNA sequences of complete chloroplast genomes in public genetic databases is constantly increasing and the data are widely used in plant phylogenetic and biotechnological research. In this work, we investigated possible inconsistencies in the stored form of publicly available chloroplast genome sequence data. The impact of these inconsistencies on the results of the phylogenetic analysis was investigated and the bioinformatic solution to identify and correct inconsistencies was implemented. The whole procedure was demonstrated using five plant families (Apiaceae, Asteraceae, Campanulaceae, Lamiaceae and Rosaceae) as examples. chloroplast genome genome assembly standardization cyclic shift inversion bioinformatika kloroplasti genom sekvenciranje genoma filogenetske analize filogenetske metode true false true Angleški jezik Slovenski jezik Članek v reviji 2022-03-30 10:56:49 2022-03-30 10:56:50 2022-09-14 04:14:56 0000-00-00 00:00:00 2021 0 0 14 str. iss. 7, art. 1360 Vol. 10 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 602 2223-7747 10.3390/plants10071360 69203459 RAZ_Turudic_Ante_2021.pdf RAZ_Turudic_Ante_2021.pdf 1 B7D0458E485C74F09C71EA779FEC6513 cd172530da8ecad1e77b97dc54a22ffe071e8df0a715c9e4b3e826493e9363d4 8cdfdd2f-b007-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=155010 https://www.mdpi.com/2223-7747/10/7/1360 1 5173f842-b007-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=155009 Biotehniška fakulteta 0 0 0