20.500.12556/RUL-135736 Effects of bioinsecticidal aegerolysin-based cytolytic complexes on non-target organisms Aegerolysin proteins ostreolysin A6 (OlyA6), pleurotolysin A2 (PlyA2) and erylysin A (EryA) produced by the mushroom genus Pleurotus bind strongly to an invertebrate-specific membrane sphingolipid, and together with a protein partner pleurotolysin B (PlyB), form transmembrane pore complexes. This pore formation is the basis for the selective insecticidal activity of aegerolysin/PlyB complexes against two economically important coleopteran pests: the Colorado potato beetle and the western corn rootworm. In this study, we evaluated the toxicities of these aegerolysin/PlyB complexes using feeding tests with two ecologically important non-target arthropod species: the woodlouse and the honey bee. The mammalian toxicity of the EryA/PlyB complex was also evaluated after intravenous administration to mice. None of the aegerolysin/PlyB complexes were toxic against woodlice, but OlyA6/PlyB and PlyA2/PlyB were toxic to honeybees, with 48 h mean lethal concentrations (LC$_{50}$) of 0.22 and 0.39 mg/mL, respectively, in their food. EryA/PlyB was also tested intravenously in mice up to 3 mg/kg body mass, without showing toxicity. With no toxicity seen for EryA/PlyB for environmentally beneficial arthropods and mammals at the tested concentrations, these EryA/PlyB complexes are of particular interest for development of new bioinsecticides for control of selected coleopteran pests. adverse effects aegerolysins Apis mellifera bioinsecticides erylysin A MACPF protein non-target organisms oyster mushroom Porcellio scaber toxicity stranski učinki egerolizini Apis mellifera bioinsekticidi erilizin A protein z domeno MACPF netarčni organizmi bukov ostrigar Porcellio scaber toksičnost true false true Angleški jezik Slovenski jezik Članek v reviji 2022-03-30 09:52:09 2022-03-30 09:52:10 2022-09-14 04:14:54 0000-00-00 00:00:00 2021 0 0 13 str. iss. 7, art. 457 Vol. 13 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577 2072-6651 10.3390/toxins13070457 68710147 RAZ_Panevska_Anastasija_2021.pdf RAZ_Panevska_Anastasija_2021.pdf 1 95CE3A5D7445D6D22AC957446A37C9C4 bed92895b6201b3da4a2508ba7d77b9c1c140ec83f54d7c8133069d5c37118b8 7ca6d336-affe-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=155000 https://www.mdpi.com/2072-6651/13/7/457 1 49570bf1-affe-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154999 Biotehniška fakulteta Veterinarska fakulteta 0 0 0