20.500.12556/RUL-135720 Large-scale transcriptomics-driven approach revealed overexpression of CRNDE as a poor survival prognosis biomarker in glioblastoma Glioblastoma is the most common and malignant brain malignancy worldwide, with a 10-year survival of only 0.7%. Aggressive multimodal treatment is not enough to increase life expectancy and provide good quality of life for glioblastoma patients. In addition, despite decades of research, there are no established biomarkers for early disease diagnosis and monitoring of patient response to treatment. High throughput sequencing technologies allow for the identification of unique molecules from large clinically annotated datasets. Thus, the aim of our study was to identify significant molecular changes between short- and long-term glioblastoma survivors by transcriptome RNA sequencing profiling, followed by differential pathway-activation-level analysis. We used data from the publicly available repositories The Cancer Genome Atlas (TCGA; number of annotated cases = 135) and Chinese Glioma Genome Atlas (CGGA; number of annotated cases = 218), and experimental clinically annotated glioblastoma tissue samples from the Institute of Pathology, Faculty of Medicine in Ljubljana corresponding to 2-58 months overall survival (n = 16). We found one differential gene for long noncoding RNA CRNDE whose overexpression showed correlation to poor patient OS. Moreover, we identified overlapping sets of congruently regulated differential genes involved in cell growth, division, and migration, structure and dynamics of extracellular matrix, DNA methylation, and regulation through noncoding RNAs. Gene ontology analysis can provide additional information about the function of protein- and nonprotein-coding genes of interest and the processes in which they are involved. In the future, this can shape the design of more targeted therapeutic approaches. glioblastoma noncoding RNA long-term survival CRNDE RNA sequencing glioblastom nekodirajoča RNA dolgoročno preživetje true false true Angleški jezik Slovenski jezik Članek v reviji 2022-03-29 14:36:35 2022-03-29 14:36:40 2022-09-14 04:14:50 0000-00-00 00:00:00 2021 0 0 18 str. iss. 14, art. 3419 Vol. 13 Jul. 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616-006 2072-6694 10.3390/cancers13143419 69980163 RAZ_Sorokin_Maxim_2021.pdf RAZ_Sorokin_Maxim_2021.pdf 1 FDB705B42BE8A778F9BDFFACC6367079 d90734342c3a4a21d3d0cb52efb4e2532693394d244a0abfed687e044b71513e 1dfc3265-af5d-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154978 https://www.mdpi.com/2072-6694/13/14/3419 1 de41d09d-af5c-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154977 Medicinska fakulteta 0 0 0