20.500.12556/RUL-135711 Detection of antibodies against tick-borne encephalitis virus in zoo animals using non-invasive blood sampling with medicinal leeches (Hirudo medicinalis) Reports on non-invasive blood sampling are limited, and there are only a few studies on using kissing bugs (Reduviidae) and medicinal leeches (Hirudo medicinalis) for hematology and biochemistry testing in various zoo animal species. The aim of this study was to evaluate the usefulness of non-invasive blood sampling with medicinal leeches for arbovirus epidemiological investigations in various animal species from one zoo collection. Medicinal leeches were manually applied on 35 animals of 11 species. Control blood samples were obtained by venipuncture of the jugular vein. Antibodies to tick-borne encephalitic virus (TBEV) were detected by using the immunoenzymatic method or an immunofluorescent assay (IFAT), depending on the animal species. One of the 35 animals (2.9%) was seropositive (Ovis aries), whereas the rest of the samples were seronegative in both methods of sampling (non-invasive by leeches vs. invasive by venipuncture). Blood sampling using medicinal leeches showed promising results. It is likely a good alternative to other more complex and invasive methods, and it can provide significant advancement in blood sampling for preventive medicine and epidemiological studies in zoo animals. infectious disease monitoring animal welfare epidemiology communicable diseases encephalitozoonosis prevention control true false true Angleški jezik Ni določen Članek v reviji 2022-03-29 12:18:44 2022-03-29 12:18:45 2022-09-14 04:14:46 0000-00-00 00:00:00 2021 0 0 6 str. iss. 8, art. 952 Vol. 10 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 636.09:616.9 2076-0817 10.3390/pathogens10080952 71927811 RAZ_Kvapil_Pavel_2021.pdf RAZ_Kvapil_Pavel_2021.pdf 1 E8915BC9B75E4ED7766BCC471E2302AA 945857f32d3f59b45b3d624816977c8d562a1ec4fe63b1e6360b41b83f3f41db d4552034-af49-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154964 https://www.mdpi.com/2076-0817/10/8/952 1 99d35735-af49-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154963 Medicinska fakulteta Veterinarska fakulteta 0 0 0