20.500.12556/RUL-135710 The elucidation of the molecular mechanism of the extrusion process Extrusion is a popular method for producing homogenous population of unilamellar liposomes. The technique relies on forcing a lipid suspension through cylindrical pores in a polycarbonate membrane. The quantification of the extrusion and/or recalibration processes make possible the acquisition of experimental data, which can be correlated with the mechanical properties of the lipid bilayer. In this work, the force needed for the extrusion process was correlated with the mechanical properties of a lipid bilayer derived from other experiments. Measurements were performed using a home-made dedicated device capable of maintaining a stable volumetric flux of a liposome suspension through well-defined pores and to continuously measure the extrusion force. Based on the obtained results, the correlation between the lipid bilayer bending rigidity and extrusion force was derived. Specifically, it was found that the bending rigidity of liposomes formed from well-defined lipid mixtures agrees with data obtained by others using flicker-noise spectroscopy or micromanipulation. The other issue addressed in the presented studies was the identification of molecular mechanisms leading to the formation of unilamellar vesicles in the extrusion process. Finally, it was demonstrated that during the extrusion, lipids are not exchanged between vesicles, i.e., vesicles can divide but no membrane fusion or lipid exchange between bilayers was detected. liposomes extrusion lipid bilayer membrane mechanics bending rigidity liposomi ekstruzija lipidni dvosloj membranska mehanika upogibna togost true false true Angleški jezik Slovenski jezik Članek v reviji 2022-03-29 12:00:42 2022-03-29 12:00:43 2022-09-14 04:14:46 0000-00-00 00:00:00 2021 0 0 20 str. iss. 15, art. 4278 Vol. 14 Aug. 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577 1996-1944 10.3390/ma14154278 72250627 RAZ_Doskocz_Joanna_2021.pdf RAZ_Doskocz_Joanna_2021.pdf 1 C68A2CD204F7DE504949C7AA80601F7D 47fed1e0b9f8ce47ca2ef7101e8835ae425ab757b8395cf4f79bf759b107d2cb 5bfc44f5-af47-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154962 https://www.mdpi.com/1996-1944/14/15/4278 1 14def0ae-af47-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154961 Fakulteta za elektrotehniko Medicinska fakulteta 0 0 0