20.500.12556/RUL-135663 Attachment of cancer urothelial cells to the bladder epithelium occurs on uroplakin-negative cells and is mediated by desmosomal and not by classical cadherins Urinary bladder cancer is often multifocal; however, the intraluminal dissemination of the urothelial cancer cells is poorly understood. The involvement of N-cadherin in the adhesion of the cancer urothelial cells to the urothelium had not previously been studied. Therefore, we herein explore the possibility of the intraluminal dissemination of the urothelial cancer cells by evaluating the role of classical cadherins in the adhesion of urothelial cancer cells to the urothelium. We used E-cadherin negative T24 cells and established a T24 Ncad$^{low}$ cell line with an additionally decreased expression of N-cadherin in the plasma membrane and a decreased secretion of proform of metalloproteinase 2. The labelled T24 and T24 Ncad$^{low}$ cells were seeded onto urothelial in vitro models. After 24 h in co-culture, unattached cancer cells were rinsed and urothelia with attached cancer urothelial cells were processed for fluorescence and electron microscopy. Both the T24 and T24 Ncad$^{low}$ cells attached to the urothelium, yet only to the uroplakin-negative urothelial cells. The ultrastructural analysis showed that T24 and T24 Ncad$^{low}$ cells adhere to poorly differentiated urothelial cells by desmosomes. To achieve this, they first disrupt tight junctions of superficial urothelial cells. This study indicates that the lack of E-cadherin expression and decreased expression of N-cadherin in the plasma membrane of T24 cells does not interfere with their adhesion to the urothelium; therefore, our results suggest that intraluminal dissemination of cancer urothelial cells along the urothelium occurs on uroplakin-negative cells and is desmosome-mediated. bladder cancer urothelial in vitro models electron microscopy N-cadherin desmosomes rak mehurja urotelijski in vitro modeli elektronska mikroskopija true false true Angleški jezik Slovenski jezik Članek v reviji 2022-03-24 07:54:32 2022-03-24 07:54:38 2022-09-14 04:14:31 0000-00-00 00:00:00 2021 0 0 18 str. iss. 11, art. 5565 Vol. 22 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616.6 1422-0067 10.3390/ijms22115565 65359619 RAZ_Dragin_Jerman_Urska_2021.pdf RAZ_Dragin_Jerman_Urska_2021.pdf 1 F51702AE676449373D67287D33AC1419 1a494aa854ee1cc9c6c35255b2399833aec573a6bf661d060fe3694db32409af 9535f87b-ab3f-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154881 https://www.mdpi.com/1422-0067/22/11/5565 1 44679576-ab3f-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154880 Medicinska fakulteta Fakulteta za elektrotehniko 0 0 0