20.500.12556/RUL-135655 How cancer cells invade bladder epithelium and form tumors the mouse bladder tumor model as a model of tumor recurrence in patients Non-muscle-invasive bladder cancer is the most common form of bladder cancer. The main problem in managing bladder tumors is the high recurrence after the transurethral resection of bladder tumors (TURBT). Our study aimed to examine the fate of intravesically applied cancer cells as the implantation of cancer cells after TURBT is thought to be a cause of tumor recurrence. We established an orthotopic mouse bladder tumor model with MB49-GFP cancer cells and traced them during the first three days to define their location and contacts with normal urothelial cells. Data were obtained by Western blot, immunolabeling, and light and electron microscopy. We showed that within the first two hours, applied cancer cells adhered to the traumatized epithelium by cell projections containing α3β1 integrin on their tips. Cancer cells then migrated through the epithelium and on day 3, they reached the basal lamina or even penetrated it. In established bladder tumors, E-cadherin and desmoplakin 1/2 were shown as feasible immunohistochemical markers of tumor margins based on the immunolabeling of various junctional proteins. Altogether, these results for the first time illustrate cancer cell implantation in vivo mimicking cellular events of tumor recurrence in bladder cancer patients. mouse bladder tumor model urinary bladder MB49 cancer cells bladder tumor urothelium intercellular junctions junctional protein model tumorja mišičnega mehurja tumor mehurja MB49 rakave celice true false true Angleški jezik Slovenski jezik Članek v reviji 2022-03-23 15:19:30 2022-03-23 15:19:35 2022-09-14 04:14:29 0000-00-00 00:00:00 2021 0 0 20 str. iss. 12, art. 6328 Vol. 22 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616-006 1422-0067 10.3390/ijms22126328 67627523 RAZ_Erman_Andreja_2021.pdf RAZ_Erman_Andreja_2021.pdf 1 259DD5F148171FC6CB6E1F6445514018 291c01c4b4e5d14daea7a2f6370f131210669ae28d66c19a06709e97b111f72e 749340a4-aab4-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154871 https://www.mdpi.com/1422-0067/22/12/6328 1 428eeefe-aab4-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154870 Medicinska fakulteta Fakulteta za elektrotehniko 0 0 0