20.500.12556/RUL-135603 Urinary extracellular vesicles and their miRNA cargo in patients with Fabry nephropathy Current biomarkers of Fabry nephropathy lack sensitivity in detecting early kidney damage and do not predict progression of nephropathy. Urinary extracellular vesicles (uEVs) and their molecular cargo could reflect early changes in renal impairment as they are secreted by the cells lining the urinary tract. We aimed to conduct a proof-of-concept study to investigate whether analysis of uEV characteristics and expression of uEV-derived microRNAs (miRNAs) could be applicable in studies to predict the development and progression of nephropathy in Fabry disease. A total of 20 Fabry patients were divided into two groups, depending on the presence of nephropathy. Chronological urine samples collected during 10-year follow-up were used for uEVs isolation with size exclusion chromatography. Nanoparticle tracking analysis was used to determine concentration and size of uEVs. We evaluated the expression of five uEV-derived miRNAs by qPCR (miR-23a-3p, miR-29a-3p, miR-30b-5p, miR-34a-5p, miR-200a-3p). There was no difference in the concentration and size of uEVs between patients with and without nephropathy at last follow-up or longitudinally. However, we found increased expression of miR-29a-3p and miR-200a-3p in uEVs isolated from chronological samples of patients with Fabry nephropathy. This may indicate an attempt by the organism to prevent the progression of renal damage leading to end-stage renal disease as previously reported in type 1 diabetes. In addition, we found an increased expression of miR-30b-5p in the 10-year period in uEVs of patients without renal dysfunction. miR-30b-5 was reported to have a protective role in podocyte injury and may possibly be important in Fabry nephropathy. These findings indicate that uEVs and their molecular cargo could be a promising target of studies focusing on elucidation of Fabry nephropathy. Nevertheless, total concentration and size of uEVs were neither indicative of the presence nor progression of Fabry nephropathy, while the role of the analyzed miRNAs in Fabry nephropathy progression was merely indicated and needs further in-depth studies. Fabry nephropathy urinary extracellular vesicles miRNA expression biomarker NTA Fabry disease lysosomal storage disease Fabryjeva nefropatija urinski zunajcelični vezikli izražanje miRNA true false true Angleški jezik Slovenski jezik Članek v reviji 2022-03-22 08:54:12 2022-03-22 08:54:17 2022-09-14 04:14:13 0000-00-00 00:00:00 2021 0 0 15 str. iss. 7, art. 1057 Vol. 12 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616.6 2073-4425 10.3390/genes12071057 70049795 RAZ_Levstek_Tina_2021.pdf RAZ_Levstek_Tina_2021.pdf 1 5E2A8DBBBEA0907EFA7CC4A7477835E5 3a1868e498efaced4e631801063a193bc698d740a9742c269a6f253762464082 ac4d8d25-a9b5-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154771 https://www.mdpi.com/2073-4425/12/7/1057 1 4630d8fb-a9b5-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154770 Medicinska fakulteta 0 0 0