20.500.12556/RUL-135599 Stability-indicating analytical approach for stability evaluation of lactoferrin Lactoferrin is a multifunctional iron-binding glycoprotein in milk. Due to its potential for the treatment of various diseases, interest in products containing lactoferrin is increasing. However, as a protein, it is prone to degradation, which critically affects the quality of products. Therefore, the main purpose of our work was to develop a stability-indicating analytical approach for stability evaluation of lactoferrin. We were focused on two complementary methods: reversed-phase and size-exclusion chromatography. The stability-indicating nature of the selected methods was confirmed. They were successfully validated by following the ICH guidelines and applied to preliminary lactoferrin stability studies. Up to three degradation products, as well as aggregates and fragments of lactoferrin, were detected in various samples using complementary reversed-phase and size-exclusion chromatographic methods. The analytical approach was additionally extended with three spectroscopic techniques (absorbance, intrinsic fluorescence, and bicinchoninic acid method), which may provide valuable complementary information in some cases. The presented analytical approach allows the stability evaluation of lactoferrin in various samples, including the ability to detect differences in its degradation mechanisms. Furthermore, it has the potential to be used for the quality control of products containing lactoferrin. lactoferrin stability HPLC-UV spectroscopic methods stability-indicating approach proteins quality control proteini stabilnost laktoferin nadzor kakovosti spektroskopska analiza true false true Angleški jezik Slovenski jezik Članek v reviji 2022-03-22 08:20:32 2022-03-22 08:20:39 2023-03-15 09:30:04 0000-00-00 00:00:00 2021 0 0 24 str. iss. 7, art. 1065 Vol. 13 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 66.022.362:535.33/.34:615 1999-4923 10.3390/pharmaceutics13071065 70061059 RAZ_Osel_Nika_2021.pdf RAZ_Osel_Nika_2021.pdf 1 FD146D2737653AF059D224697919DEBF 097ecca6b2e44e120c034ae0b30b25e7cbd5d05df2b3b3d75b14f1a4d898738e d0c2349e-a9b0-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154765 https://www.mdpi.com/1999-4923/13/7/1065 1 9398367a-a9b0-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154764 Fakulteta za farmacijo 0 0 0