20.500.12556/RUL-135591 Identification of novel RNA binding proteins influencing circular RNA expression in hepatocellular carcinoma Circular RNAs (circRNAs) are increasingly recognized as having a role in cancer development. Their expression is modified in numerous cancers, including hepatocellular carcinoma (HCC); however, little is known about the mechanisms of their regulation. The aim of this study was to identify regulators of circRNAome expression in HCC. Using publicly available datasets, we identified RNA binding proteins (RBPs) with enriched motifs around the splice sites of differentially expressed circRNAs in HCC. We confirmed the binding of some of the candidate RBPs using ChIP-seq and eCLIP datasets in the ENCODE database. Several of the identified RBPs were found to be differentially expressed in HCC and/or correlated with the overall survival of HCC patients. According to our bioinformatics analyses and published evidence, we propose that NONO, PCPB2, PCPB1, ESRP2, and HNRNPK are candidate regulators of circRNA expression in HCC. We confirmed that the knocking down the epithelial splicing regulatory protein 2 (ESRP2), known to be involved in the maintenance of the adult liver phenotype, significantly changed the expression of candidate circRNAs in a model HCC cell line. By understanding the systemic changes in transcriptome splicing, we can identify new proteins involved in the molecular pathways leading to HCC development and progression. hepatocellular carcinoma (HCC) circular RNA (circRNA) identification of novel proteins RNA binding proteins (RBPs) ESRP2 hepatocelularni karcinom krožna RNA identifikacija novih proteinov true false true Angleški jezik Slovenski jezik Članek v reviji 2022-03-21 14:30:27 2022-03-21 14:30:31 2022-09-14 04:14:08 0000-00-00 00:00:00 2021 0 0 14 str. iss. 14, art. 7477 Vol. 22 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616-006 1422-0067 10.3390/ijms22147477 70157827 RAZ_Razpotnik_Rok_2021.pdf RAZ_Razpotnik_Rok_2021.pdf 1 85D532E263FFFC18B3867AE7FE421DFC 0efd2305497c3a4bee4909f100087f69b9efe1c9c226e3c8c07c73d6568986ad 43c7fe6c-a91b-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154751 https://www.mdpi.com/1422-0067/22/14/7477 1 15529384-a91b-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154750 Medicinska fakulteta Biotehniška fakulteta 0 0 0