20.500.12556/RUL-135577 Dirofilaria repens in dogs and humans in Slovenia Introduction: The prevalence of Dirofilaria repens in dogs in countries bordering Slovenia ranges from 1.5% to 47.3%. The aim of this study was to estimate its prevalence in Slovenian dogs and to present the cases of dirofilariasis diagnosed in humans from 2010 to 2020. Material and Methods: Epidemiological data were collected and blood samples were taken from 465 dogs older than one year and born in Slovenia. A real-time PCR was performed on all samples to detect filarioid DNA, and a D. repens- and D. immitis-specific real-time PCR was performed on positive samples. Blood samples from 446 dogs were tested for Dirofilaria spp. using a modified Knott’s test. Human cases were diagnosed from histological sections of excised subcutaneous nodules. Descriptive statistics were used to characterise the samples. The one-sample nonparametric chi-squared test was used to assess whether categories of a variable were equally distributed. Results: Three dogs’ samples tested positive for D. repens using the species-specific real-time PCR, while D. immitis DNA was not detected. The modified Knott’s test was positive in two of the three PCR-positive dogs, two of which had never travelled outside Slovenia’s borders. Four human patients with D. repens dirofilariasis were diagnosed. Since their travel history was unknown, autochthonous transmission could not be confirmed. Conclusion: Our study demonstrated a 0.64% prevalence of D. repens infection in dogs in Slovenia. Two cases could be autochthonous. dirofilariasis D. repens prevalence dogs humans true false true Angleški jezik Ni določen Članek v reviji 2022-03-21 10:23:56 2022-03-21 10:23:57 2022-09-14 04:14:04 0000-00-00 00:00:00 2022 0 0 7 str. no. 1 Vol. 66 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616 2450-7393 10.2478/jvetres-2022-0008 101420291 RAZ_Kotnik_Tina_2022.pdf RAZ_Kotnik_Tina_2022.pdf 1 DB722E4CA5783431388CB4E42B735614 880cabd92c9f48daf14fb8a2ad34f20fc293ba02fc4707f6a2a74c97a8a4a576 581d52c6-a8fa-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154732 https://www.sciendo.com/article/10.2478/jvetres-2022-0008 0 46a6c9ec-a8fa-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154731 Veterinarska fakulteta Medicinska fakulteta 0 0 0