20.500.12556/RUL-135541 Novel Siphoviridae bacteriophages infecting Bacteroides uniformis contain diversity generating retroelement Intestinal phages are abundant and important components of gut microbiota, yet the isolated and characterized representatives that infect abundant gut bacteria are sparse. Here we describe the isolation of human intestinal phages infecting Bacteroides uniformis. Bacteroides is one of the most common bacterial groups in the global human gut microbiota; however, to date not many Bacteroides specific phages are known. Phages isolated in this study belong to a novel viral genus, Bacuni, within the Siphoviridae family. Their genomes encode diversity-generating retroelements (DGR), which were shown in other bacteriophages to promote phage adaptation to rapidly changing environmental conditions and to broaden their host range. Three isolated phages showed 99.83% genome identity but one of them infected a distinct B. uniformis strain. The tropism of Bacuni phages appeared to be dependent on the interplay of DGR mediated sequence variations of gene encoding putative phage fimbrial tip proteins and mutations in host genes coding for outer-membrane proteins. We found prophages with up to 85% amino acid similarity over two-thirds of the Bacuni phage genome in the B. acidifaciens and Prevotella sp. genomes. Despite the abundance of Bacteroides within the human microbiome, we found Bacuni phages only in a limited subset of published gut metagenomes. gut Bacteroides virome prophage diversity-generating retroelement mikrobiologija bakterije Bacteroides uniformis Siphoviridae bakteriofagi črevesje mikrobiota true false true Angleški jezik Slovenski jezik Članek v reviji 2022-03-18 08:33:07 2022-03-18 08:33:08 2022-09-14 04:13:54 0000-00-00 00:00:00 2021 0 0 16 str. iss. 5, art. 892 Vol. 9 Apr. 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579 2076-2607 10.3390/microorganisms9050892 61197059 RAZ_Hedzet_Stina_2021.pdf RAZ_Hedzet_Stina_2021.pdf 1 2DC48DC16CE337ECFB727AE96150DD3A 554c906f6f2a9d0ec3a31efe41dcad927ee5df1d6548c48d3b53ff7a2aa0e16b e557e69f-a68d-11ec-960c-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154668 https://www.mdpi.com/2076-2607/9/5/892 1 a8dd528c-a68d-11ec-960c-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154667 Biotehniška fakulteta 0 0 0