20.500.12556/RUL-135450 Biocompatibility study of a new dental cement based on hydroxyapatite and calcium silicates focus on liver, kidney, and spleen tissue effects The effects of a new material based on hydroxyapatite and calcium silicates, named ALBO-MPCA, were investigated on the liver, kidney and spleen. The material was administrated orally for 120 days in an in vivo model in Wistar rats, and untreated animals served as a control. Hematological and biochemical blood parameters were analyzed. Qualitative histological analysis of tissues, change in mitotic activity of cells, and histological characteristics was conducted, as well as quantitative stereological analysis of parenchymal cells, blood sinusoids, and connective tissues. Additionally, the protein expressions of Ki67 and CD68 markers were evaluated. Histological analysis revealed no pathological changes after the tested period. It showed the preservation of the architecture of blood sinusoids and epithelial cells and the presence of mitosis. Additionally, the significantly increased number of the Ki67 in the presence of ALBO-MPCA confirmed the proliferative effect of the material noticed by stereological analysis, while immunoreactive CD68 positive cells did not differ between groups. The study showed non-toxicity of the tested material based on the effects on the hematological, biochemical, and observed histological parameters; in addition, it showed evidence of its biocompatibility. These results could be the basis for further steps toward the application of tested materials in endodontics. biocompatibility calcium silicates dental cement hydroxyapatite subchronic toxicity true false true Angleški jezik Ni določen Članek v reviji 2022-03-15 13:41:35 2022-03-15 13:41:35 2022-09-14 04:13:24 0000-00-00 00:00:00 2021 0 0 15 str. iss. 11, art. 5468 Vol. 22 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577 1422-0067 10.3390/ijms22115468 64625411 RAZ_Paras_Smiljana_2021.pdf RAZ_Paras_Smiljana_2021.pdf 1 48744105618DB4D21A52D20549CCCA8A 0ab90ffa85c01aa17d0fe7e6b81c3e657ddcc3c5997b9131dfa21b63242da87c 85c3d22b-a45d-11ec-8556-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154539 https://www.mdpi.com/1422-0067/22/11/5468 1 45df27bb-a45d-11ec-8556-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154538 Biotehniška fakulteta 0 0 0