20.500.12556/RUL-135373 Cold plasma affects germination and fungal community structure of buckwheat seeds Crop seeds are frequently colonised by fungi from the field or storage places. Some fungi can cause plant diseases or produce mycotoxins, compromising the use of seeds as seeding material, food or feed. We have investigated the effects of cold plasma (CP) on seed germination and diversity of seed-borne fungi in common and Tartary buckwheat. The seeds were treated with CP for 15, 30, 45, 60, 90, and 120 s in a low-pressure radiofrequency system using oxygen as the feed gas. The fungi from the seed surface and fungal endophytes were isolated using potato dextrose agar plates. After identification by molecular methods, the frequency and diversity of fungal strains were compared between CP treated and chemically surface-sterilised (30% of H$_2$O$_2$) seeds. CP treatments above 60 s negatively affected the germination of both buckwheat species. A significant reduction in fungal frequency and diversity was observed after 90 s and 120 s in common and Tartary buckwheat, respectively. The filamentous fungi of genera Alternaria and Epicoccum proved to be the most resistant to CP. The results of our study indicate that CP treatment used in our study may be applicable in postharvest and food production, but not for further seed sowing. cold plasma seeds grains plants common buckwheat Tartary buckwheat fungi decontamination Alternaria Didymella Epicoccum hladna plazma semena zrna navadna ajda tatarska ajda glive dekontaminacija true false true Angleški jezik Slovenski jezik Članek v reviji 2022-03-09 10:35:10 2022-03-09 10:35:11 2022-09-14 04:13:04 0000-00-00 00:00:00 2021 0 0 18 str. iss. 5, art. 851 Vol. 10 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 581.1 2223-7747 10.3390/plants10050851 61089795 RAZ_Mravlje_Jure_2021.pdf RAZ_Mravlje_Jure_2021.pdf 1 3384B5FE2C07FF073550CCD8FAC3E13A 3166aff4dbf7e6224b20ebe2797406d98253acb9fb93a669d224cec546647f2e 37269ca9-9f8c-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154414 https://www.mdpi.com/2223-7747/10/5/851 1 ef3d67c8-9f8b-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154413 Biotehniška fakulteta 0 0 0