20.500.12556/RUL-135352 Osamitev, identifikacija in karakterizacija gliv iz avtomehanične delavnice magistrsko delo Isolation, identification and characterisation of fungi from car service shop magistrski študij - 2. stopnja Vse večje število avtomobilov in drugih motornih vozil povečuje onesnaženost ozračja, talne, gozdne in vodne ekosisteme z ogljikovodiki. Z namenom najti glivne seve, ki bi jih lahko uporabili v bioremediacijskih postopkih, smo preverili njihovo prisotnost v avtomobilih, in sicer v delih, ki pridejo v stik z bencinom, oljem ali nafto. Vzorčili smo čistilce olja, goriva, ter odprtino za dovod goriva. Poleg tega smo v avtomehanični delavnici vzorčili odtočni jašek interne kanalizacije in talne oljne madeže. Iz vzorcev sem s pomočjo različnih izolacijskih tehnik osamil 45 izolatov gliv. Identificirali smo jih s pomočjo nukleotidih zaporedij izbranih molekularnih označevalcev za posamezen rod. Seve smo uvrstili v 13 rodov, 11 sevov v deblo Ascomycota (Acremonium, Alternaria, Aureobasidium, Candida, Cladosporium, Coniochaeta, Exophiala, Geotrichum, Meyerozyma, Penicilium, Pleurostoma), 2 seva v deblo Basidiomycota (Cystobasidium in Rhodotorula). Več kot polovica izolatov (26) je pripadala rodovom Aureobasidium, Candida, Meyerozyma in Rhodotorula. Vse izolirane glive smo testirali na zmožnost razgradnje toluena, mineralnega olja in n-heksadekana. Vsi izolati so rastli na toluenu, medtem ko je bila rast na n-heksadekanu in mineralnem olju omejena na manj kot 50%, in sicer na rodove Meyerozyma, Acremonium, Penicilium, Cladosporum in Exophiala. The increasing number of motor vehicles is causing a lot of air pollution with hydrocarbons. The pollution spreads as well to the forests, rivers, water and ground ecosystems. We decided to investigate the vehicle's internal combustion engine and its parts, for potential presence of fungi that could be used as bioremediation agents. Oil filters, fuel filters, hole for refuelling, car service workshop's drainage system and oil stains on the floor from car service workshop were analyzed in a laboratory for the presence of fungi. We used different isolation techniques and with the aid of molecular markers we identified 45 fungal species belonging to 13 genera. Acremonium, Alternaria, Aureobasidium, Candida, Cladosporium, Coniochaeta, Exophiala, Geotrichum, Meyerozyma, Penicilium, Pleurostoma, Cystobasidium and Rhodotorula. All of the isolates were tested for growth on selected hydrocarbons as sole carbon sources. All of the isolates grew in toluene atmosphere, while less than 50% (Meyerozyma, Acremonium, Penicilium, Cladosporum and Exophiala) grew on mineral oil and even less on n-hexadecane (Meyerozyma, Acremonium, Penicilium and Coniochaeta). biologija glive avtomehanična delavnica ogljikovodiki aromatske spojine toluen n-heksadekan Biology fungi car service hydrocarbons aromatic compounds toluene n-hexadecane true false false [T. Gnidovec] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2022-03-09 07:15:08 2022-03-09 07:15:21 2022-09-14 04:12:56 0000-00-00 00:00:00 2022 0 Ljubljana 0 Ljubljana XI, 51 f. 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 582.28(043.2) 203334 103833859 22243.pdf 22243.pdf 1 B98A8B2E0EE5F8D62AD9BD129D50A987 d56cf04f6a7ada50af4cd8b926b24ed96abe601f87dfb3647369aebc1795dc18 0638d716-9f70-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154384 Biotehniška fakulteta 0 0 0