20.500.12556/RUL-135349 Water pores in planar lipid bilayers at fast and slow rise of transmembrane voltage Basic understanding of the barrier properties of biological membranes can be obtained by studying model systems, such as planar lipid bilayers. Here, we study water pores in planar lipid bilayers in the presence of transmembrane voltage. Planar lipid bilayers were exposed to fast and slow linearly increasing voltage and current signals. We measured the capacitance, breakdown voltage, and rupture time of planar lipid bilayers composed of 1-pamitoyl 2-oleoyl phosphatidylcholine (POPC), 1-pamitoyl 2-oleoyl phosphatidylserine (POPS), and a mixture of both lipids in a 1:1 ratio. Based on the measurements, we evaluated the change in the capacitance of the planar lipid bilayer corresponding to water pores, the radius of water pores at membrane rupture, and the fraction of the area of the planar lipid bilayer occupied by water pores. The estimated pore radii determining the rupture of the planar lipid bilayer upon fast build-up of transmembrane voltage are 0.101 nm, 0.110 nm, and 0.106 nm for membranes composed of POPC, POPS, and POPC:POPS, respectively. The fraction of the surface occupied by water pores at the moment of rupture of the planar lipid bilayer is in the range of 0.1–1.8%. planar lipid bilayer capacitance voltage breakdown water pores hydrophobic pores ravninski lipidni dvosloj kapacitivnost porušitvena napetost vodne pore hidrofobne pore true false true Angleški jezik Slovenski jezik Članek v reviji 2022-03-08 15:01:00 2022-03-08 15:01:01 2022-09-14 04:12:55 0000-00-00 00:00:00 2021 0 0 16 str. iss. 4, art. 263 Vol. 11 Apr. 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577 2077-0375 10.3390/membranes11040263 59163139 RAZ_Macek_Lebar_Alenka_2021.pdf RAZ_Macek_Lebar_Alenka_2021.pdf 1 E5E237D688404A86A76FDC2F9876D986 a7a795fe919df0bf91572297f7d848008992f54dd05ae2ee3668890932b149f9 1e50c922-9ee8-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154380 https://www.mdpi.com/2077-0375/11/4/263 1 e86a5916-9ee7-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154379 Fakulteta za elektrotehniko 0 0 0