20.500.12556/RUL-135331 Bacterial cell wall material properties determine E. coli resistance to sonolysis The applications of bacterial sonolysis in industrial settings are plagued by the lack of the knowledge of the exact mechanism of action of sonication on bacterial cells, variable effectiveness of cavitation on bacteria, and inconsistent data of its efficiency. In this study we have systematically changed material properties of E. coli cells to probe the effect of different cell wall layers on bacterial resistance to ultrasonic irradiation (20 kHz, output power 6,73 W, horn type, 3 mm probe tip diameter, 1 ml sample volume). We have determined the rates of sonolysis decay for bacteria with compromised major capsular polymers, disrupted outer membrane, compromised peptidoglycan layer, spheroplasts, giant spheroplasts, and in bacteria with different cell physiology. The non-growing bacteria were 5-fold more resistant to sonolysis than growing bacteria. The most important bacterial cell wall structure that determined the outcome during sonication was peptidoglycan. If peptidoglycan was remodelled, weakened, or absent the cavitation was very efficient. Cells with removed peptidoglycan had sonolysis resistance equal to lipid vesicles and were extremely sensitive to sonolysis. The results suggest that bacterial physiological state as well as cell wall architecture are major determinants that influence the outcome of bacterial sonolysis. viability bacteria Escherichia coli sonication cell envelope bakterijske celice Escherichia coli celična ovojnica liza celic ultrazvočna sonikacija sonoliza true false true Angleški jezik Slovenski jezik Članek v reviji 2022-03-08 09:50:12 2022-03-08 09:50:17 2022-09-14 04:12:49 0000-00-00 00:00:00 2022 0 0 10 str. art. 105919 Vol. 83 Feb. 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579.23:577.352:544.57 1350-4177 10.1016/j.ultsonch.2022.105919 93971971 RAZ_Pandur_Ziga_2022.pdf RAZ_Pandur_Ziga_2022.pdf 1 3CE9B1279904050F1D454A9C8D6A5786 1b6aaee2573aa0a69ed4e72bc7fe251bacc84d6f4f22f995f82a9dae0669ecca b570f45e-9ebd-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154347 https://www.sciencedirect.com/science/article/pii/S1350417722000128?via%3Dihub 1 7f954e2f-9ebc-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154344 Biotehniška fakulteta Fakulteta za strojništvo 0 0 0