20.500.12556/RUL-135330 Kin discrimination drives territorial exclusion during Bacillus subtilis swarming and restrains exploitation of surfactin Swarming is the collective movement of bacteria across a surface. It requires the production of surfactants (public goods) to overcome surface tension and provides an excellent model to investigate bacterial cooperation. Previously, we correlated swarm interaction phenotypes with kin discrimination between B. subtilis soil isolates, by showing that less related strains form boundaries between swarms and highly related strains merge. However, how kin discrimination affects cooperation and territoriality in swarming bacteria remains little explored. Here we show that the pattern of surface colonization by swarming mixtures is influenced by kin types. Closely related strain mixtures colonize the surface in a mixed swarm, while mixtures of less related strains show competitive exclusion as only one strain colonizes the surface. The outcome of nonkin swarm expansion depends on the initial ratio of the competing strains, indicating positive frequency-dependent competition. We find that addition of surfactin (a public good excreted from cells) can complement the swarming defect of nonkin mutants, whereas close encounters in nonkin mixtures lead to territorial exclusion, which limits the exploitation of surfactin by nonkin nonproducers. The work suggests that kin discrimination driven competitive territorial exclusion may be an important determinant for the success of cooperative surface colonization. Bacillus subtilis swaraming social interactions surfactin biofilms microbial ecology Bacillus subtilis rojenje socialne interakcije surfaktin true false true Angleški jezik Slovenski jezik Članek v reviji 2022-03-08 09:41:48 2022-03-08 09:41:53 2023-05-25 15:24:01 0000-00-00 00:00:00 2022 0 0 Str. 833–841 iss. 3 Vol. 16 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579.22/.26:579.852.11 1751-7362 10.1038/s41396-021-01124-4 80968963 RAZ_Kraigher_Barbara_2022.pdf RAZ_Kraigher_Barbara_2022.pdf 1 EC65EC333B0EDD3D178113294D93E166 51fc2ede6cddcd7e9557b7730daaf43170b0a28a45722877529fe6c43be6bc96 d2fab229-9ebb-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154342 https://www.nature.com/articles/s41396-021-01124-4 0 d8e6513c-9ebb-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154343 Biotehniška fakulteta 0 0 0