20.500.12556/RUL-135303 Novel protocol for acute in situ ecotoxicity test using native crustaceans applied to groundwater ecosystems Current standardized laboratory test protocols use model species that have limitations to accurately assess native species responses to stressors. We developed and tested a novel acute in situ protocol for testing field-collected organisms. We used Asellus aquaticus and NaCl as a reference toxicant to test for the effects of location (laboratory vs. in situ), medium (synthetic vs. field water), substrate (presence vs. absence), and protocol replicability. We further tested the protocol using groundwater-adapted isopods: Proasellus assaforensis for the effect of location, P. cavaticus of medium and P. lusitanicus of substrate. Our results showed that A. aquaticus’ lethality obtained with the novel acute in situ protocol did not significantly differ from those from laboratory testing. However, laboratory tested P. assaforensis showed a higher sensitivity, suggesting that its acclimation to laboratory conditions might have pernicious effects. A. aquaticus and P. cavaticus showed a higher mortality using synthetic medium in situ and under laboratory conditions, which overestimated the stressor’s effect. Besides, substrate use had no significant effect. The novel acute in situ protocol allows the use of native species under realistic scenarios. It is particularly well adapted for assessing the risk of groundwater ecosystems but it can be applied to a wide range of ecosystems. groundwater ecology groundwater ecotoxicology stygofauna in situ exposure site-specific assessment ekologija ekotoksikologija podtalnica true false true Angleški jezik Slovenski jezik Članek v reviji 2022-03-07 12:24:06 2022-03-07 12:24:07 2022-09-14 04:12:41 0000-00-00 00:00:00 2021 0 0 13 str. iss. 8, art. 1132 Vol. 13 Apr. 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 628.112:502/504 2073-4441 10.3390/w13081132 64665347 RAZ_Castano-Sanchez_Andrea_2021.pdf RAZ_Castano-Sanchez_Andrea_2021.pdf 1 73FD127C98CE8D59EA5C3AE0A68434C2 9d1bd7cc7d9ad9762f09efa5209bf1349c12e3d8a86f314fefa1ab5c15302f5e 124a2bc0-9e09-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154297 https://www.mdpi.com/2073-4441/13/8/1132 0 d3984aa0-9e08-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154296 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0