20.500.12556/RUL-135300 Escherichia coli affects expression of circadian clock genes in human hepatoma cells Recent research has indicated that dysbiosis of the gut microbiota can lead to an altered circadian clock of the mammalian host. Herein we developed an original system that allows real-time circadian studies of human HepG2 hepatoma cells co-cultured with bacteria. The HepG2 cells with stably integrated firefly luciferase reporter under the control of PERIOD2 promoter were co-cultured with E. coli strains isolated from human fecal samples from healthy individuals. The two E. coli strains differ in the phylogenetic group and the number of ExPEC virulence-associated genes: BJ17 has only two, and BJ23 has 15 of 23 tested. In the first 24 h, the E. coli BJ17 affected the HepG2 circadian clock more than BJ23. Cosinor analysis shows a statistically significant change in the amplitude of PER1 and 2 and the phase advance of PER3. A high percentage of necrotic and apoptotic cells occurred at 72 h, while a correlation between the number of ExPEC genes and the influence on the HepG2 core clock gene expression was observed. Our study reveals that the E. coli genetic background is important for the effect on the mammalian circadian clock genes, indicating possible future use of probiotic E. coli strains to influence the host circadian clock. intestinal microbiota Escherichia coli circadian clock circadian rhythm HepG2 true false true Angleški jezik Ni določen Članek v reviji 2022-03-07 11:15:49 2022-03-07 11:15:50 2022-09-14 04:12:40 0000-00-00 00:00:00 2021 0 0 14 str. iss. 4, art. 869 Vol. 9 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579 2076-2607 10.3390/microorganisms9040869 61091843 RAZ_Kovac_Ursa_2021.pdf RAZ_Kovac_Ursa_2021.pdf 1 C8ABE3130883CB159CB8C85ABA364CC6 f71958ed800a4bbc0e8d8917996ce707cafd5ca91241c724e0971b532574add1 932bd036-9dff-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154290 https://www.mdpi.com/2076-2607/9/4/869 1 498920d8-9dff-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154289 Medicinska fakulteta Biotehniška fakulteta Fakulteta za računalništvo in informatiko 0 0 0