20.500.12556/RUL-135293 Karakterizacija tvorbe gvanozinskih nanožic z metodami sipanja svetlobe Characterization of formation of guanosine nanowires by light scattering methods Optični metodi dinamično sipanje svetlobe (DLS) in diferenčna dinamična mikroskopija (DDM) sta primerni eksperimentalni tehniki za zaznavanje submikronskih delcev v različnih suspenzijah. V našem primeru so to vodne suspenzije z gvaninom bogatih oligonukleotidov DNK, ki ob prisotnosti primerne koncentracije kationov, kot sta recimo Na+ ali K+, tvorijo daljše strukture imenovane G-žičke. Metodi sta osnovani na sipanju svetlobe in izračunu avtokorelacijske funkcije intenzitete svetlobe zaznane na fotopomnoževalki ali zelo hitri kameri. Kljub temu, da je princip merjenja pri omenjenih metodah različen, se rezultati - na podlagi primernega matematičnega modela - med seboj dopolnjujejo in so primerljivi. Z navedenima metodama smo določili difuzijske koeficiente sipalcev suspenzij, iz katerih smo ugotovili, katere mutacije začetnega oligonukleotida d(G2AG4 AG2) tvorijo G-žičke ter kolikšna je dolžina teh žičk. Zaznali smo G-žičke različnih velikosti, katerih efektivne dolžine so v razponu od L min ⠼ 20 nm do L max ⠼ 140 nm. Optical methods, such as dynamic light scattering (DLS) and differential dynamic microscopy (DDM) are suitable experimental techniques for detecting submicron particles in different suspensions. In our case, these are aqueous suspensions of guanine-rich oligonucleotides that in the presence of a suitable concentration of cations, such as Na+ or K+, form longer structures called G-wires. The methods are based on light scattering and the calculation of the autocorrelation function of the perceived light intensity on a photomultiplier or a high-speed camera. Despite the fact, that the principle of measurement in the two mentioned methods is different, the results - based on a suitable mathematical model - complement each other and are comparable. With both methods, we determined the diffusion coefficients of the suspension, from which we deduced, which mutation of the initial oligonucleotide d(G2AG4AG2) form G-wires and what is the length of these wires. We detected G-wires of different sizes, whose effective lengths range from L min ⠼ 20 nm to L max ⠼ 140 nm. oligonukleotidi DNK G-kvarteti G-kvadrupleksi G-žičke Brownovo gibanje dinamično sipanje svetlobe diferenčna dinamična mikroskopija DNA oligonucleotides G-quartets G-quadruplexes G-wires Brownian motion dynamic light scattering differential dynamic microscopy true false false Slovenski jezik Angleški jezik Magistrsko delo/naloga 2022-03-06 08:15:02 2022-03-06 08:15:14 2022-09-14 04:12:37 0000-00-00 00:00:00 2022 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 123061 99751683 4338.pdf 4338.pdf 1 62DA63BBAAC2343169B4ADDC7EF18524 e9b72a5fc784d8aeb773a79aef4b57bde7bbdc7b7f121c1e1172f44bea5450fb e5745143-9d1c-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154276 Fakulteta za matematiko in fiziko 0 0 0