20.500.12556/RUL-135243 The antibacterial activity of human amniotic membrane against multidrug-resistant bacteria associated with urinary tract infections new insights from normal and cancerous urothelial models Urinary tract infections (UTIs) represent a serious global health issue, especially due to emerging multidrug-resistant UTI-causing bacteria. Recently, we showed that the human amniotic membrane (hAM) could be a candidate for treatments and prevention of UPEC and Staphylococcus aureus infections. However, its role against multidrug-resistant bacteria, namely methicillin-resistant S. aureus (MRSA), extended-spectrum beta-lactamases (ESBL) producing Escherichia coli and Klebsiella pneumoniae, vancomycin-resistant Enterococci (VRE), carbapenem-resistant Acinetobacter baumannii, and Pseudomonas aeruginosa has not yet been thoroughly explored. Here, we demonstrate for the first time that the hAM homogenate had antibacterial activity against 7 out of 11 tested multidrug-resistant strains, the greatest effect was on MRSA. Using novel approaches, its activity against MRSA was further evaluated in a complex microenvironment of normal and cancerous urinary bladder urothelia. Even short-term incubation in hAM homogenate significantly decreased the number of bacteria in MRSA-infected urothelial models, while it did not affect the viability, number, and ultrastructure of urothelial cells. The hAM patches had no antibacterial activity against any of the tested strains, which further exposes the importance of the hAM preparation. Our study substantially contributes to basic knowledge on the antibacterial activity of hAM and reveals its potential to be used as an antibacterial agent against multidrug-resistant bacteria. amniotic membrane homogenate MRSA antibiotic resistance multidrug-resistant bacteria antimicrobial activity electron microscopy urothelial cells urinary bladder homogenat amnijske membrane MRSA odpornost na antibiotike true false true Angleški jezik Slovenski jezik Članek v reviji 2022-03-02 11:49:07 2022-03-02 11:49:11 2022-09-14 04:12:23 0000-00-00 00:00:00 2021 0 0 21 str. iss. 2, art. 218 Vol. 9 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616.6 2227-9059 10.3390/biomedicines9020218 52599811 RAZ_Zeleznik_Ramuta_Taja_2021.pdf RAZ_Zeleznik_Ramuta_Taja_2021.pdf 1 3FB4B44C65E0F7C4814930C1C8101456 9ef62d8ce65f6429b6df7003d6cd78f3f147d17a98d44628be6b85a72a0b9087 69c74d9d-9a16-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154188 https://www.mdpi.com/2227-9059/9/2/218 1 208b611a-9a16-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154187 Medicinska fakulteta Biotehniška fakulteta 0 0 0