20.500.12556/RUL-135230 Stressor-dependant changes in immune parameters in the terrestrial isopod crustacean, Porcellio scaber a focus on nanomaterials We compared the changes of selected immune parameters of Porcellio scaber to different stressors. The animals were either fed for two weeks with Au nanoparticles (NPs), CeO$_2$ NPs, or Au ions or body-injected with Au NPs, CeO$_2$ NPs, or lipopolysaccharide endotoxin. Contrary to expectations, the feeding experiment showed that both NPs caused a significant increase in the total haemocyte count (THC). In contrast, the ion-positive control resulted in a significantly decreased THC. Additionally, changes in phenoloxidase (PO)-like activity, haemocyte viability, and nitric oxide (NO) levels seemed to depend on the stressor. Injection experiments also showed stressor-dependant changes in measured parameters, such as CeO$_2$ NPs and lipopolysaccharide endotoxin (LPS), caused more significant responses than Au NPs. These results show that feeding and injection of NPs caused an immune response and that the response differed significantly, depending on the exposure route. We did not expect the response to ingested NPs, due to the low exposure concentrations (100 µg/g dry weight food) and a firm gut epithelia, along with a lack of phagocytosis in the digestive system, which would theoretically prevent NPs from crossing the biological barrier. It remains a challenge for future research to reveal what the physiological and ecological significance is for the organism to sense and respond, via the immune system, to ingested foreign material. gold nanoparticles cerium nanoparticles woodlice immune response haemocyte imunski odziv kopenski raki nanodelci spremembe imunskih parametrov true false true Angleški jezik Slovenski jezik Članek v reviji 2022-03-02 08:28:10 2022-03-02 08:28:11 2022-09-14 04:12:18 0000-00-00 00:00:00 2021 0 0 18 str. iss. 4, art. 934 Vol. 11 Apr. 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577 2079-4991 10.3390/nano11040934 59056899 RAZ_Mayall_Craig_2021.pdf RAZ_Mayall_Craig_2021.pdf 1 5F6B10972AD02317BA30B20828359848 52c9c70893196f082b46a6352b5e360b0be8d588e62978eb72abc941144990b8 6b10bc95-99fa-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154167 https://www.mdpi.com/2079-4991/11/4/934 1 0c9dffc6-99fa-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154165 Biotehniška fakulteta 0 0 0