20.500.12556/RUL-135182 Core RNA interference genes involved in miRNA and ta-siRNA biogenesis in hops and their expression analysis after challenging with Verticillium nonalfalfae RNA interference is an evolutionary conserved mechanism by which organisms regulate the expression of genes in a sequence-specific manner to modulate defense responses against various abiotic or biotic stresses. Hops are grown for their use in brewing and, in recent years, for the pharmaceutical industry. Hop production is threatened by many phytopathogens, of which Verticillium, the causal agent of Verticillium wilt, is a major contributor to yield losses. In the present study, we performed identification, characterization, phylogenetic, and expression analyses of three Argonaute, two Dicer-like, and two RNA-dependent RNA polymerase genes in the susceptible hop cultivar Celeia and the resistant cultivar Wye Target after infection with Verticillium nonalfalfae. Phylogeny results showed clustering of hop RNAi proteins with their orthologues from the closely related species Cannabis sativa, Morus notabilis and Ziziphus jujuba which form a common cluster with species of the Rosaceae family. Expression analysis revealed downregulation of argonaute 2 in both cultivars on the third day post-inoculation, which may result in reduced AGO2-siRNA-mediated posttranscriptional gene silencing. Both cultivars may also repress ta-siRNA biogenesis at different dpi, as we observed downregulation of argonaute 7 in the susceptible cultivar on day 1 and downregulation of RDR6 in the resistant cultivar on day 3 after inoculation. Humulus lupulus Verticillium nonalfalfae fungal infection RNA interference plant–pathogen interactions small RNA Humulus lupulus Verticillium nonalfalfae verticilijska uvelost glivična okužba interferenca RNA interakcija rastlina-patogen true false true Angleški jezik Slovenski jezik Članek v reviji 2022-02-28 09:58:01 2022-02-28 09:58:03 2022-09-14 04:12:01 0000-00-00 00:00:00 2021 0 0 16 str. iss. 8, art. 4224 Vol. 22 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577.2 1422-0067 10.3390/ijms22084224 60443139 RAZ_Kunej_Urban_2021.pdf RAZ_Kunej_Urban_2021.pdf 1 F667F671CDB121FB69B5EB041CFFD306 2f5360c4662362a87137ee1bade2ae490b30d545672e5bffcac8e5f43febb922 9aefe3e2-9874-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154074 https://www.mdpi.com/1422-0067/22/8/4224 1 462d6e25-9874-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154073 Biotehniška fakulteta 0 0 0