20.500.12556/RUL-135042 Novakomyces olei sp. nov., the first member of a novel Taphrinomycotina lineage Taphrinomycotina is the smallest subphylum of the phylum Ascomycota. It is an assemblage of distantly related early diverging lineages of the phylum, comprising organisms with divergent morphology and ecology; however, phylogenomic analyses support its monophyly. In this study, we report the isolation of a yeast strain, which could not be assigned to any of the currently recognised five classes of Taphrinomycotina. The strain of the novel budding species was recovered from extra virgin olive oil and characterised phenotypically by standard methods. The ultrastructure of the cell wall was investigated by transmission electron microscopy. Comparisons of barcoding DNA sequences indicated that the investigated strain is not closely related to any known organism. Tentative phylogenetic placement was achieved by maximum-likelihood analysis of the D1/D2 domain of the nuclear LSU rRNA gene. The genome of the investigated strain was sequenced, assembled, and annotated. Phylogenomic analyses placed it next to the fission Schizosaccharomyces species. To accommodate the novel species, Novakomyces olei, a novel genus Novakomyces, a novel family Novakomycetaceae, a novel order Novakomycetales, and a novel class Novakomycetes is proposed as well. Functional analysis of genes missing in N. olei in comparison to Schizosaccharomyces pombe revealed that they are biased towards biosynthesis of complex organic molecules, regulation of mRNA, and the electron transport chain. Correlating the genome content and physiology among species of Taphrinomycotina revealed some discordance between pheno- and genotype. N. olei produced ascospores in axenic culture preceded by conjugation between two cells. We confirmed that N. olei is a primary homothallic species lacking genes for different mating types. genome sequence phylogenomics functional gene analysis novel yeast species novel class olive oil kvasovke taksonomija kvasovk Novakomyces olei filogenomika true false true Angleški jezik Slovenski jezik Članek v reviji 2022-02-18 10:58:54 2022-02-18 10:58:55 2022-09-14 04:11:21 0000-00-00 00:00:00 2021 0 0 19 str. iss. 2, art. 301 Vol. 9 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579.8:582.282.23 2076-2607 10.3390/microorganisms9020301 49990915 RAZ_Cadez_Neza_2021.pdf RAZ_Cadez_Neza_2021.pdf 1 E97D0BF77E6A9F750E3B4715601FC87B e256b5945f91f10d224456ec54394f7f3408157a59e36aabaf655efa5ac9853e 650e261f-90a1-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=153883 https://www.mdpi.com/2076-2607/9/2/301 1 2330bcd0-90a1-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=153882 Biotehniška fakulteta 0 0 0