20.500.12556/RUL-135038 Adipose tissue gene expression of entire male, immunocastrated and surgically castrated pigs Differences in adipose tissue deposition and properties between pig male sex categories, i.e., entire males (EM), immunocastrates (IC) and surgical castrates (SC) are relatively well-characterized, whereas the underlying molecular mechanisms are still not fully understood. To gain knowledge about the genetic regulation of the differences in adipose tissue deposition, two different approaches were used: RNA-sequencing and candidate gene expression by quantitative PCR. A total of 83 differentially expressed genes were identified between EM and IC, 15 between IC and SC and 48 between EM and SC by RNA-sequencing of the subcutaneous adipose tissue. Comparing EM with IC or SC, upregulated genes related to extracellular matrix dynamics and adipogenesis, and downregulated genes involved in the control of lipid and carbohydrate metabolism were detected. Differential gene expression generally indicated high similarity between IC and SC as opposed to EM, except for several heat shock protein genes that were upregulated in EM and IC compared with SC. The candidate gene expression approach showed that genes involved in lipogenesis were downregulated in EM compared with IC pigs, further confirming RNA-sequencing results. pigs adipose tissue entire males immunocastration surgical castration RNA-sequencing expression prašiči maščobno tkivo samci imunokastracija kirurška kastracija true false true Angleški jezik Slovenski jezik Članek v reviji 2022-02-18 08:46:37 2022-02-18 08:46:39 2023-05-04 13:57:02 0000-00-00 00:00:00 2021 0 0 18 str. iss. 4, art. 1768 Vol. 22 Feb. 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 636.4 1422-0067 10.3390/ijms22041768 50997507 RAZ_Poklukar_Klavdija_2021.pdf RAZ_Poklukar_Klavdija_2021.pdf 1 BB1E24475161C37A503936D3E8CC4453 ea08782b0689a5652c156b626db480c705bc37a90fb8a3af09e7cd1a568e2260 f1f0b3bd-908e-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=153874 https://www.mdpi.com/1422-0067/22/4/1768 1 a910c163-908e-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=153873 Veterinarska fakulteta 0 0 0