20.500.12556/RUL-135037 Identification and validation of new cancer stem cell-related genes and their regulatory microRNAs in colorectal cancerogenesis Significant progress has been made in the last decade in our understanding of the pathogenetic mechanisms of colorectal cancer (CRC). Cancer stem cells (CSC) have gained much attention and are now believed to play a crucial role in the pathogenesis of various cancers, including CRC. In the current study, we validated gene expression of four genes related to CSC, L1TD1, SLITRK6, ST6GALNAC1 and TCEA3, identified in a previous bioinformatics analysis. Using bioinformatics, potential miRNA-target gene correlations were prioritized. In total, 70 formalin-fixed paraffin-embedded biopsy samples from 47 patients with adenoma, adenoma with early carcinoma and CRC without and with lymph node metastases were included. The expression of selected genes and microRNAs (miRNAs) was evaluated using quantitative PCR. Differential expression of all investigated genes and four of six prioritized miRNAs (hsa-miR-199a-3p, hsa-miR-335-5p, hsa-miR-425-5p, hsa-miR-1225-3p, hsa-miR-1233-3p and hsa-miR-1303) was found in at least one group of CRC cancerogenesis. L1TD1, SLITRK6, miR-1233-3p and miR-1225-3p were correlated to the level of malignancy. A negative correlation between miR-199a-3p and its predicted target SLITRK6 was observed, showing potential for further experimental validation in CRC. Our results provide further evidence that CSC-related genes and their regulatory miRNAs are involved in CRC development and progression and suggest that some them, particularly miR-199a-3p and its SLITRK6 target gene, are promising for further validation in CRC. colorectal cancer differentially expressed genes cancer stem cells qPCR kolorektalni rak različno izraženi geni matične celice raka true false true Angleški jezik Slovenski jezik Članek v reviji 2022-02-18 08:29:12 2022-02-18 08:29:18 2022-09-14 04:11:19 0000-00-00 00:00:00 2021 0 0 20 str. iss. 2, art. 179 Vol. 9 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616-006 2227-9059 10.3390/biomedicines9020179 53489155 RAZ_Urh_Kristian_2021.pdf RAZ_Urh_Kristian_2021.pdf 1 2877AF26AD413F5C8A8E0E40202ABFFE 18cfa0dc15cbb36ddd3190f93dceed47e0862de84ee70447f7e8347a9c09e9e9 9b4d7c6c-908c-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=153872 https://www.mdpi.com/2227-9059/9/2/179 0 3c79af00-908c-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=153871 Medicinska fakulteta 0 0 0