20.500.12556/RUL-134922 Copper(II) and zinc(II) complexes with the clinically used fluconazole comparison of antifungal activity and therapeutic potential Copper(II) and zinc(II) complexes with clinically used antifungal drug fluconazole (fcz), {[CuCl$_2$(fcz)$_2$]·5H$_2$O}$_n$, 1, and {[ZnCl$_2$(fcz)$_2$]·2C$_2$H$_5$OH}$_n$, 2, were prepared and characterized by spectroscopic and crystallographic methods. The polymeric structure of the complexes comprises four fluconazole molecules monodentately coordinated via the triazole nitrogen and two chlorido ligands. With respect to fluconazole, complex 2 showed significantly higher antifungal activity against Candida krusei and Candida parapsilosis. All tested compounds reduced the total amount of ergosterol at subinhibitory concentrations, indicating that the mode of activity of fluconazole was retained within the complexes, which was corroborated via molecular docking with cytochrome P450 sterol 14α-demethylase (CYP51) as a target. Electrostatic, steric and internal energy interactions between the complexes and enzyme showed that 2 has higher binding potency to this target. Both complexes showed strong inhibition of C. albicans filamentation and biofilm formation at subinhibitory concentrations, with 2 being able to reduce the adherence of C. albicans to A549 cells in vitro. Complex 2 was able to reduce pyocyanin production in Pseudomonas aeruginosa between 10% and 25% and to inhibit its biofilm formation by 20% in comparison to the untreated control. These results suggest that complex 2 may be further examined in the mixed Candida-P. aeruginosa infections. copper(II) complex zinc(II) complex fluconazole antifungal agents anti-biofilm activity baker cink kovinski kompleksi true false true Angleški jezik Slovenski jezik Članek v reviji 2022-02-11 09:27:11 2022-02-11 09:27:12 2022-09-14 04:10:41 0000-00-00 00:00:00 2021 0 0 20 str. iss. 1, art. 24 Vol. 14 Jan. 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 546.47:546.562 1424-8247 10.3390/ph14010024 45141251 RAZ_Stevanovic_Nevena_Lj._2021.pdf RAZ_Stevanovic_Nevena_Lj._2021.pdf 1 0078BB20E341D3446360BAF87B999C5F 6d71faf8b7d52497bed04a031988e12a7696db689b6ef1efb8c45e7d9480f8c9 90b8cadb-8b14-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=153666 https://www.mdpi.com/1424-8247/14/1/24 0 2dea3fbf-8b14-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=153665 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0