20.500.12556/RUL-134855 Effect of hydrolyzable tannins on glucose-transporter expression and their bioavailability in pig small-intestinal 3D cell model Intestinal transepithelial transport of glucose is mediated by glucose transporters, and affects postprandial blood-glucose levels. This study investigates the effect of wood extracts rich in hydrolyzable tannins (HTs) that originated from sweet chestnut (Castanea sativa Mill.) and oak (Quercus petraea) on the expression of glucose transporter genes and the uptake of glucose and HT constituents in a 3D porcine-small-intestine epithelial-cell model. The viability of epithelial cells CLAB and PSI exposed to different HTs was determined using alamarBlue$^®$. qPCR was used to analyze the gene expression of SGLT1, GLUT2, GLUT4, and POLR2A. Glucose uptake was confirmed by assay, and LC–MS/ MS was used for the analysis of HT bioavailability. HTs at 37 µg/mL were found to adversely affect cell viability and downregulate POLR2A expression. HT from wood extract Tanex at concentrations of 4 µg/mL upregulated the expression of GLUT2, as well as glucose uptake at 1 µg/mL. The time-dependent passage of gallic acid through enterocytes was influenced by all wood extracts compared to gallic acid itself as a control. These results suggest that HTs could modulate glucose uptake and gallic acid passage in the 3D cell model. hydrolyzable tannin glucose glucose transporters porcine epithelial cell line tannin bioavailability glucose transport glukoza tanini biokemija true false true Angleški jezik Slovenski jezik Članek v reviji 2022-02-04 13:20:01 2022-02-04 13:20:02 2022-09-14 04:10:21 0000-00-00 00:00:00 2021 0 0 17 str. iss. 2, art. 345 Vol. 26 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577 1420-3049 10.3390/molecules26020345 46450435 RAZ_Brus_Maksimiljan_2021.pdf RAZ_Brus_Maksimiljan_2021.pdf 1 0712C3EAE92A1F1BE63A67C12B1F9874 842b7ed1b6f0d688166f654e9a9a38c1c6bec7017fd91a864352fa8f168cf974 e6b3e1e7-85b4-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=153557 https://www.mdpi.com/1420-3049/26/2/345 1 8ec53348-85b4-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=153556 Veterinarska fakulteta 0 0 0