20.500.12556/RUL-134835 The structure and function of paraoxonase-1 and its comparison to paraoxonase-2 and -3 Serum paraoxonase-1 (PON1) is the most studied member of the group of paraoxonases (PONs). This enzyme possesses three enzymatic activities: lactonase, arylesterase, and paraoxonase activity. PON1 and its isoforms play an important role in drug metabolism as well as in the prevention of cardiovascular and neurodegenerative diseases. Although all three members of the PON family have the same origin and very similar amino acid sequences, they have different functions and are found in different locations. PONs exhibit substrate promiscuity, and their true physiological substrates are still not known. However, possible substrates include homocysteine thiolactone, an analogue of natural quorum-sensing molecules, and the recently discovered derivatives of arachidonic acid—bioactive δ-lactones. Directed evolution, site-directed mutagenesis, and kinetic studies provide comprehensive insights into the active site and catalytic mechanism of PON1. However, there is still a whole world of mystery waiting to be discovered, which would elucidate the substrate promiscuity of a group of enzymes that are so similar in their evolution and sequence yet so distinct in their function. paraoxonase lactonase oxidative stress PON1 PON2 PON3 arylesterase organophosphate structure kinetic atherosclerosis paraoksonaza laktonaza oksidativni stres true false true Angleški jezik Slovenski jezik Članek v reviji 2022-02-03 16:38:51 2022-02-03 16:38:53 2022-09-14 04:10:13 0000-00-00 00:00:00 2020 0 0 20 str. iss. 24, art. 5980 Vol. 25 Dec. 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577 1420-3049 10.3390/molecules25245980 43242755 RAZ_Taler-Vercic_Ajda_2020.pdf RAZ_Taler-Vercic_Ajda_2020.pdf 1 32A76A196C0635BF3051D478095891A5 9eb88ea8d51111729a555f8e15d5e389f9552f0f8bd1579da1813cd6635defa3 65ccc77f-8508-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=153518 https://www.mdpi.com/1420-3049/25/24/5980 1 2c4f6155-8507-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=153517 Medicinska fakulteta 0 0 0