20.500.12556/RUL-134820 The central region of testican-2 forms a compact core and promotes cell migration Testicans are modular proteoglycans of the extracellular matrix of various tissues where they contribute to matrix integrity and exert cellular effects like neurite outgrowth and cell migration. Using testican-2 as a representative member of the family, we tackle the complete lack of general structural information and structure–function relationship. First, we show using isothermal titration calorimetry and modeling that extracellular calcium-binding domain (EC) has only one active calcium-binding site, while the other potential site is inactive, and that testican-2 is within extracellular matrix always in the calcium-loaded form. Next, we demonstrate using various prediction methods that N- and C-terminal regions plus interdomain connections are flexible. We support this by small-angle X-ray-scattering analysis of C-terminally truncated testican-2, which indicates that the triplet follistatin-EC-thyroglobulin domain forms a moderately compact core while the unique N-terminal is disordered. Finally, using cell exclusion zone assay, we show that it is this domain triplet that is responsible for promoting cell migration and not the N- and C-terminal regions. testican SPOCK calcium-binding structural model cell migration testikan SPOCK vezava kalcijevih ionov model strukture celična migracija true false true Angleški jezik Slovenski jezik Članek v reviji 2022-02-03 11:31:26 2022-02-03 11:31:27 2023-03-21 09:05:06 0000-00-00 00:00:00 2020 0 0 17 str. iss. 24, art. 9413 Vol. 21 Dec. 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577.112.85 1661-6596 10.3390/ijms21249413 42261507 RAZ_Krajnc_Anja_2020.pdf RAZ_Krajnc_Anja_2020.pdf 1 F05FC5BE7D6A0BDC3F8A790EEAE14A57 a53629016bd99b479849c2f91eb9bdd50a77f1d40a191c28618e216899997878 9df1632b-84dc-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=153487 https://www.mdpi.com/1422-0067/21/24/9413 0 3953a9ac-84dc-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=153486 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0