20.500.12556/RUL-134776 Plasma extracellular vesicle characteristics correlate with tumor differentiation and predict overall survival in patients with pancreatic ductal adenocarcinoma undergoing surgery with curative intent Better preoperative characterization of patients with pancreatic ductal adenocarcinoma (PDAC) would aid in treatment optimization. Extracellular vesicles (EV) are promising, largely unexplored biomarkers in PDAC. This study aimed to evaluate if plasma EV characteristics are associated with PDAC clinical characteristics and overall survival (OS). The prospective cohort included 34 PDAC patients undergoing surgery with curative intent. Patient data and plasma samples were collected preoperatively, intraoperatively and one month postoperatively. Small plasma EV (sEV) concentration and size were determined by nanoparticle-tracking analysis. A Mann-Whitney test, Spearman's rho and Cox regression were used in statistical analysis. Preoperatively, patients with poorly differentiated tumors had significantly larger plasma sEVs when compared to patients with well/moderately differentiated tumors (mean diameter 176.9 vs. 149.2 nm, p = 0.021), the sEV size even enabling discrimination of the two groups (AUC = 0.742, 95% CI = 0.560-0.923). Plasma sEV characteristics were also a predictor of OS in multivariable analysis. Patients with a more than 33.8% increase in sEV concentration after one month had 7.2 months shorter median OS (p = 0.002), while patients with a more than 28.0% decrease in sEV size had 9.2 months shorter median OS (p = 0.045). Plasma sEV concentration and size correlate with tumor differentiation and may predict OS in PDAC patients. In the future, plasma sEV characteristics could contribute to improved patient stratification for optimized treatment. pancreatic cancer tumor differentiation overall survival extracellular vesicles nanoparticle-tracking analysis plasma biomarkers liquid biopsy rak trebušne slinavke diferenciacija tumorja splošno preživetje true false true Angleški jezik Slovenski jezik Članek v reviji 2022-02-01 12:38:00 2022-02-01 12:38:04 2022-09-14 04:09:51 0000-00-00 00:00:00 2021 0 0 12 str. iss. 2, art. 77 Vol. 11 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616-006 2075-4426 10.3390/jpm11020077 49258243 RAZ_Badovinac_David_2021.pdf RAZ_Badovinac_David_2021.pdf 1 7BA76BC225EBC111F81D81AA1310C9BB 9c5a6389bf3ab9efb00d0856cbd4cfcfed3d95818de1ec0f3e95b0546921d079 71dcb928-8353-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=153409 https://www.mdpi.com/2075-4426/11/2/77 1 34651414-8353-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=153408 Medicinska fakulteta 0 0 0