20.500.12556/RUL-134698 Comparison of Campylobacter jejuni slaughterhouse and surface-water isolates indicates better adaptation of slaughterhouse isolates to the chicken host environment Campylobacter jejuni is an emerging food-borne pathogen that poses a high risk to human health. Knowledge of the strain source can contribute significantly to an understanding of this pathogen, and can lead to improved control measures in the food-processing industry. In this study, slaughterhouse and surface-water isolates of C. jejuni were characterized and compared in terms of their antimicrobial resistance profiles and adhesion to stainless steel and chicken skin. Resistance of C. jejuni biofilm cells to benzalkonium chloride and Satureja montana ethanolic extract was also tested. The data show that the slaughterhouse isolates are more resistant to ciprofloxacin, and adhere better to stainless steel at 42 °C, and at 37 °C in 50% chicken juice. Additionally, biofilm cells of the isolate with the greatest adhesion potential (C. jejuni S6) were harvested and tested for resistance to S. montana ethanolic extract, benzalkonium chloride, and erythromycin; and for efflux-pump activity, as compared to their planktonic cells. The biofilm cells showed increased resistance to both S. montana ethanolic extract and erythromycin, and increased efflux-pump activity. These data indicate adaptation of C. jejuni slaughterhouse isolates to the chicken host, as well as increased biofilm cell resistance due to increased efflux-pump activity. Campylobacter jejuni slaughterhouse isolates surface-water isolates biofilm resistance efflux pump activity patogeni mikroorganizmi Campylobacter jejuni biofilmi klavniški sevi protimikrobno delovanje rastlinski izvlečki kraški šetraj Satureja montana etanolni izvlečki benzalkonijev klorid izlivne črpalke true false true Angleški jezik Slovenski jezik Članek v reviji 2022-01-26 14:55:59 2022-01-26 14:56:00 2022-09-13 03:56:37 0000-00-00 00:00:00 2020 0 0 15 str. iss. 11, art. 1693 Vol. 8 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579.22/.26:547.9:615.281 2076-2607 10.3390/microorganisms8111693 35369987 RAZ_Simunovic_Katarina_2020.pdf RAZ_Simunovic_Katarina_2020.pdf 1 12C21C18A581E813EBC7E64CEFA93613 09cbbcda459b75c95b48e2ce9c9596e47adf40dfa3607d262ffef73203d4497b c45614dd-7eaf-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=153277 https://www.mdpi.com/2076-2607/8/11/1693 1 7dd72293-7eaf-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=153276 Biotehniška fakulteta 0 0 0