20.500.12556/RUL-134695 Determination of genetically identical strains of four honeybee viruses in bumblebee positive samples In recent years, there has been growing evidence that certain types of honeybee viruses could be transmitted between different pollinators. Within a voluntary monitoring programme, 180 honeybee samples (Apis mellifera carnica) were collected from affected apiaries between 2007 and 2018. Also from August 2017 to August 2018, a total 148 samples of healthy bumblebees (Bombus lapidarius, B. pascuorum, B. terrestris, B. lucorum, B. hortorum, B. sylvarum, B. humilis) were collected at four different locations in Slovenia, and all samples were tested by using RT-PCR methods for six honeybee viruses. Direct sequencing of a total 158 positive samples (acute bee paralysis virus (ABPV n = 33), black queen cell virus (BQCV n = 75), sacbrood bee virus (SBV n = 25) and Lake Sinai virus (LSV n = 25)) was performed from obtained RT-PCR products. The genetic comparison of identified positive samples of bumblebees and detected honeybee field strains of ABPV, BQCV, SBV, and LSV demonstrated 98.74% to 100% nucleotide identity between both species. This study not only provides evidence that honeybees and bumblebees are infected with genetically identical or closely related viral strains of four endemically present honeybee viruses but also detected a high diversity of circulating strains in bumblebees, similar as was observed among honeybees. Important new genetic data for endemic strains circulating in honeybees and bumblebees in Slovenia are presented. honeybees bumblebees viruses sequencing transmission epidemiology bees true false true Angleški jezik Ni določen Članek v reviji 2022-01-26 14:15:37 2022-01-26 14:15:37 2022-09-13 03:56:36 0000-00-00 00:00:00 2020 0 0 16 str. iss. 11, art. 1310 Vol. 12 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 638:578 1999-4915 10.3390/v12111310 37519875 RAZ_Toplak_Ivan_2020.pdf RAZ_Toplak_Ivan_2020.pdf 1 C28449862652755ADF9DA5B807B36C56 807efeb6fedeee172cbc303a5c855361c5ad791c81203b6666d0d9d0c05b0a63 2af74664-7eaa-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=153272 https://www.mdpi.com/1999-4915/12/11/1310 0 d98a4516-7ea9-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=153271 Veterinarska fakulteta 0 0 0